Showing papers by "Ichiro Ono published in 1996"
TL;DR: Results showed that a very small amount of rhbFGF has a strong promotive effect on the osteogenetic activity of rhBMP, and exhibited significantly greater bone induction than the group that received the pellets treated with thesmall amount of RhBMP alone.
Abstract: In this study, we investigated the promotive effect of basic fibroblast growth factor (bFGF) on the osteogenetic activity of bone morphogenetic protein (BMP) used in conjunction with porous hydroxyapatite ceramic pellets. Results showed that there was extensive bone formation as early as 3 weeks after insertion in the group that received pellets treated with a relatively large amount of recombinant human (rh) BMP alone as well as in the group in which pellets were treated with a small amount of rhBMP combined with rhbFGF. Subsequently, osteogenesis within the pores of the pellets of the two groups slowly progressed over time; by 9 weeks after insertion, most of the pellet pores were filled with newly generated bone. In contrast, the group that received pellets treated with rhbFGF alone showed little osteogenesis, as was the case with the control group. The group that received the pellets treated with the small amount of rhBMP as well as the rhbFGF group exhibited significantly greater bone induction than the group that received the pellets treated with the small amount of rhBMP alone. These results indicated that a very small amount of rhbFGF has a strong promotive effect on the osteogenetic activity of rhBMP.
34 citations
TL;DR: It was postulated that the cytokines were bound to high molecular weight substances such as serum proteins in these exudates and could escape neutralization by antibodies directed against them.
Abstract: Recent studies have shown that tissue exudates, such as burn blister fluid and donor site wound fluid, have promotive effects on wound healing. In the present study, results obtained with enzyme-linked immunosorbent assays showed that at least three cytokines, transforming growth factor-beta1, transforming growth factor-alpha, and interleukin-6, exist at high concentrations in burn blister fluids. It was also found that these exudates were able to greatly enhance the rate of human fibroblast proliferation. However, neutralizing antibodies to transforming growth factor-beta1, transforming growth factor-alpha, interleukin-6, and platelet-derived growth factor failed to prevent this proliferative effect. Ultrafiltration of the exudates showed these cytokines to be in the high molecular weight fractions (30 to 300 kDa and >300 kDa) and that these fractions retained mitogenic activity toward fibroblasts. Therefore it was postulated that the cytokines were bound to high molecular weight substances such as serum proteins in these exudates and could escape neutralization by antibodies directed against them.
21 citations
TL;DR: The application of PGE1 ointment in combination with skin grafting surgery was found to improve functional and aesthetic results in the patients with DDB and DB, by minimizing the area of the donor site; it was especially useful for children with extensive burns.
20 citations
TL;DR: An operation to correct Stahl's ear, a rare but difficult to treat auricular malformation, using a conchal cartilage graft to support the area after closure of the defect.
17 citations