Showing papers by "Ie Ming Shih published in 1993"
Journal Article•
TL;DR: Results suggest that the phenotypic characteristics of nevus and melanoma cells in the dermis, i.e., proliferation and expression of tumor-associated antigens, may be due to their loss of contact with undifferentiation keratinocytes.
110 citations
TL;DR: Studies on autocrine and paracrine roles of growth factors in melanoma development and progression will require models that can account for the complex interactions between normal and malignant cells.
80 citations
TL;DR: It is shown that models of differentiation of melanoma cells can be established that help to define pathways of differentiation and 3 out of 4 cell lines were non‐tumorigenic in athymic nude mice.
Abstract: Malignant melanoma cells can differentiate spontaneously in vivo and in vitro into cells with a finite lifespan. Analysis of differentiating cells from primary melanomas in culture revealed a flat, fibroblast-like morphology and expression of the fibroblast-associated marker leucine aminopeptidase (LAP). Differentiation was also observed in a minor sub-population of permanent cell lines derived from metastatic lesions. An experimental model of melanoma cell differentiation was then developed, using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR-treated cells had a flat morphology, were contact-inhibited, had up to 20-fold increased surface area, expressed LAP, no longer proliferated anchorage-independently in soft agar, and 3 out of 4 cell lines were non-tumorigenic in athymic nude mice. Our results show that models of differentiation of melanoma cells can be established that help to define pathways of differentiation.
22 citations