Showing papers by "Imran Amin published in 2002"
TL;DR: In this article, the authors identify a highly conserved region upstream of a predicted hairpin structure in DNA beta and use primers designed to this region to amplify the full-length DNA beta component from total nucleic acid extracts.
Abstract: DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation. Sequence comparisons of the two available DNA beta sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA beta component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.
451 citations
TL;DR: To detect a begomovirus, both symptomatic and asymptomatic plants were collected from several widely separated locations in the Punjab Province, and results confirm that yellow vein disease on this weed is associated with a monopartite begmovirus and a distinct DNA β.
Abstract: The recent discovery that monopartite begomoviruses on ageratum and cotton essentially require a DNA satellite called DNA β (2,4) is leading to identification of several other hosts that have simil...
13 citations