Simon E. Bull

TL;DR: Analysis of sequences revealed a highly conserved organization for DNA beta molecules consisting of a single conserved open reading frame, an adenine-rich region, and a region of high sequence conservation [the satellite conserved region (SCR).

TL;DR: The analysis shows that DNA 1 components are associated with the majority of begomovirus-DNA beta complexes, being absent from only two of the complexes examined, both of which have their origins in Far East Asia.

TL;DR: This work has investigated the ability of CLCuD DNA β to interact with three further clones of monopartite begomoviruses, isolated from cotton, that have distinct Rep binding specificities and all three cloned viruses were capable of trans-replicating the satellite molecule and inducing CLCUD symptoms in cotton, indicating that the interaction between beggingomovirus and DNA β is relaxed in comparison to the interactionbetween DNA A and DNA B components.

TL;DR: Two abutting primer pairs are designed to conserved sequences of DNA 1 that allowed polymerase chain reaction-mediated amplification of the full-length molecule from total nucleic acid extracts produced from various host plants from geographically distinct, worldwide locations.

TL;DR: A comprehensive, reliable and improved protocol that optimizes Agrobacterium-mediated transformation of FEC from cassava model cultivar TMS60444 is presented, taking ∼6 months, that permits a finely balanced control of nutrients, hormones and antibiotics.