The silanol group and its role in liquid chromatography

Retention in reversed-phase liquid chromatography as a function of mobile-phase composition

The pH calibration procedures that lead to the different pH scales in acetonitrile−water mixtures used as mobile phases in reversed-phase liquid chromatography are discussed. Appropriate buffers of known pH value in acetonitrile−water mixtures are selected and used to establish the relationship (δ values) between the two rigorous acetonitrile−water pH scales:  and (pH measured in acetonitrile−water mixtures and referred to acetonitrile−water or water, respectively, as standard state). These δ values allow one to convert pH values measured in acetonitrile−water with electrode systems calibrated with aqueous buffers ( scale) to values, which are directly related to the thermodynamic acid−base constants. This offers an easy way to measure the pH of acetonitrile−water mobile phases and to relate this pH to the chromatographic retention of acids and bases through the thermodynamic acid−base constants. The relationships are tested for the variation of the retention of acids and bases with the pH of the mobile p...

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Retention of ionizable compounds on HPLC. 5. pH scales and the retention of acids and bases with acetonitrile-water mobile phases

https://www.compudrug.com/sites/default/files/filepicker/1/article2000_04.pdf

Optimization methods in chromatography and capillary electrophoresis.

Computerized design of separation strategies by reversed-phase liquid chromatography: development of DryLab software

Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. I. Accuracy of a theory-based model.

Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. II. Resolution as a function of simultaneous change in pH and solvent strength.

J.A. Lewis

Papers

Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. I. Accuracy of a theory-based model.

Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. II. Resolution as a function of simultaneous change in pH and solvent strength.

Initial experiments in high-performance liquid chromatographic method development II. Recommended approach and conditions for isocratic separation