Journal ArticleDOI
Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. II. Resolution as a function of simultaneous change in pH and solvent strength.
TLDR
The optimization of reversed-phase high-performance liquid chromatographic separation by the simultaneous variation of pH and solvent strength (%B) was studied for acidic and basic samples and the combination of these two variables was expected to be more useful than either variable alone.About:
This article is published in Journal of Chromatography A.The article was published on 1992-02-21. It has received 69 citations till now. The article focuses on the topics: High-performance liquid chromatography & Benzoic acid.read more
Citations
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Journal ArticleDOI
Retention in reversed-phase liquid chromatography as a function of mobile-phase composition
TL;DR: The dependence of solute retention (k′) on mobile phase composition (%B) for binary-solvent mixtures A-B is reviewed and compared with various empirical and theoretical equations that have been proposed for this relationship as discussed by the authors.
Journal ArticleDOI
Retention of ionizable compounds on HPLC. 5. pH scales and the retention of acids and bases with acetonitrile-water mobile phases
TL;DR: The use of the rigorous sspH and wspH scales allows one to explain the retention behavior of bases, which in many instances cannot be justified from the pH measurement in the ill-founded wwpH scale.
Journal ArticleDOI
Computerized design of separation strategies by reversed-phase liquid chromatography: development of DryLab software
TL;DR: The recent availability of new 32-bit programming tools allowed calculations of chromatograms to be completed more quickly so as to show peak movements which result for example from slight changes in eluent pH.
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Retention of ionizable compounds on HPLC. 4. Mobile-phase pH measurement in methanol/water
TL;DR: The retention of several compounds with acid/base behavior in a C-18 and a polymeric column with buffered methanol/water as mobile phase is related to the mobile phase pH value measured in the three pH scales, and it is demonstrated that the s(w)pH and s(s) pH scales give better relationships than the w(w]pH scale.
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Influence of mobile phase acid-base equilibria on the chromatographic behaviour of protolytic compounds.
Martí Rosés,Elisabeth Bosch +1 more
TL;DR: Since relationships between analyte retention and mobile phase pH depends also on the pKa value of the analyte, the solute pKa data in water-organic solvent mixtures more commonly used as chromatographic mobile phase are also reviewed.
References
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Journal ArticleDOI
Gradient selection in reversed-phase liquid chromatography
TL;DR: In this article, a general expression is derived for the numerical calculation of retention data for the case when the relationship between the capacity factor,k, and the mobile phase composition, φ, is known.
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Influence of organic modifiers on the rentention behaviour in reversed-phase liquid chromatography and its consequences for gradient elution
TL;DR: In this paper, an extensive study of 32 aromatic compounds in aqueous system containing 10-100% of methanol, acetonitrile and tetrahydro-furan as organic modifiers under isocratic conditions is described.
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DryLab computer simulation for high-performance liquid chromatographic method development. II. Gradient elution.
TL;DR: In this paper, computer simulation as an aid for the development of gradient high-performance liquid chromatographic methods is reviewed and several examples of its application are presented and the accuracy of such predictions is discussed.
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B and C terms in the Van Deemter equation for liquid chromatography
John H. Knox,Hugh P. Scott +1 more
TL;DR: In this article, the authors derived explicit equations for the B and C terms in the Van Deemter equation for liquid chromatography, involving diffusion coefficients in the mobile zone, the stagnant mobile phase and the stationary phase.
Journal ArticleDOI
Drylab® computer simulation for high-performance liquid chromatographic method development
TL;DR: Computer simulation (DryLab software) as an aid for the development of gradient high-performance liquid chromatographic methods is reviewed and the accuracy of such predictions is discussed.