Showing papers by "Jack A. Roth published in 1979"
TL;DR: The patency rate for saphenous vein grafts compares favorably with the reported patency for internal mammary grafts when critical factors such as vessel size and degree of stenosis are considered in bypass selection.
75 citations
TL;DR: This study shows that the ACT test did not reduce postoperative bleeding significantly when compared with the standard protocol and indicates that there is wide individual sensitivity to heparin and that significantly less protamine is required for reversal.
27 citations
TL;DR: IgG antibody was detected by immunodiffusion in the nonstimulatory extract of fresh tumor, but was not detected in the stimulatory tissue culture extracts or extracts of normal tissues from the same patient.
Abstract: SummaryExtracts of both fresh tumor and tissue culture cells propagated from these tumors were compared in a lymphocyte stimulation assay. Tissue culture lines were grown in media containing 20% fetal calf serum (FCS) and in serum-free chemically defined medium. Melanoma-associated antigens were extracted by means of 3 M KCl. Melanoma-associated antigens from two tissue culture cell lines stimulated 3H-thymidine (3H-Tdr) incorporation in lymphocytes from 7 of 17 melanoma patients, whereas no stimulation was noted in response to extracts of isologous fresh surgical specimens. The antigen solubilized from tissue culture cells demonstrated tumor-associated specificity. Stimulated 3H-Tdr incorporation was noted in lymphocytes from 17 of 38 melanoma patients, 2 of 18 patients with other cancers, and 3 of 18 normal subjects. Extracts from both FCS-grown-and chemically defined medium-grown cells were stimulatory. Fetal calf serum was detected in extracts of FCS-grown cells by complement fixation, but was not present in stimulatory concentrations. IgG antibody was detected by immunodiffusion in the nonstimulatory extract of fresh tumor, but was not detected in the stimulatory tissue culture extracts or extracts of normal tissues from the same patient.
5 citations
TL;DR: Important clinical considerations are the precise preoperative assessment of the lesion, careful planning of anesthetic management, choice of the appropriate incision, avoidance of circumferential dissection, and the construction of an anastomosis free of disruptive tension.
Abstract: Resection and end-to-end anastomosis has been effective in correcting localized tracheal obstruction. This procedure can be utilized in the definitive management of extensive tracheal lesions requiring the resection of at least eight tracheal rings. Important clinical considerations are the precise preoperative assessment of the lesion, careful planning of anesthetic management, choice of the appropriate incision, avoidance of circumferential dissection, and the construction of an anastomosis free of disruptive tension.
3 citations
TL;DR: In this article, the incorporation of 3H-labeled protein and nucleic acid precursors was measured by measuring the incorporation in lymphocyte cultures of 29 cancer patients and normal donors.
Abstract: Lymphocyte stimulation to 3 M KCl extracts of fresh human tumors was studied by measuring the incorporation of 3H-labeled protein and nucleic acid precursors. Lymphocytes from cancer patients and normal donors were incubated with autologous and allogeneic extracts. Duplicate lymphocyte cultures were labeled with 3H-leucine (3H-Leu), 3H-uridine (3H-Udr), or 3H-thymidine (3H-Tdr). All patients were sensitized to keyhole limpet hemocyanin (KLH) prior to testing. Of the 29 cancer patients tested, many demonstrated significant uptake of 3H-Udr (90%) and 3H-Leu (62%), but not 3H-Tdr (7%) in response to soluble tumor extracts. However, most patients demonstrated uptake of all three precursors in response to KLH. Lymphocytes from cancer patients did not undergo morphologic blast cell transformation in the presence of tumor extracts. Significant incorporation of 3H-Leu and 3H-Udr was seen after 24–48 h of incubation, while significant 3H-Tdr incorporation was not detected until day 5. Stimulation by KLH was significantly greater for all isotope precursors than stimulation in response to tumor extracts. Responses of lymphocytes from normal donors to tumor extracts were noted, although they occurred less frequently than in lymphocytes from cancer patients. Lymphocytes from cancer patients incorporated 3H-Leu and 3H-Udr, but only rarely incorporated 3H-Tdr in response to 3 M KCl extracts of fresh tumors.
2 citations