J
Jacques Dubochet
Researcher at University of Lausanne
Publications - 156
Citations - 16269
Jacques Dubochet is an academic researcher from University of Lausanne. The author has contributed to research in topics: Cryo-electron microscopy & Electron microscope. The author has an hindex of 60, co-authored 155 publications receiving 15503 citations. Previous affiliations of Jacques Dubochet include Rutgers University & European Bioinformatics Institute.
Papers
More filters
Journal ArticleDOI
Cryo-electron microscopy of vitrified specimens.
Jacques Dubochet,Marc Adrian,J.-J. Chang,Jean-Claude Homo,J. Lepault,Alasdair W. McDowall,Patrick Schultz +6 more
TL;DR: Water is the most abundant component of biological material, but it is systematically excluded from conventional electron microscopy, because water evaporates rapidly under the vacuum conditions of an electron microscope.
Journal ArticleDOI
Cryo-electron microscopy of viruses
TL;DR: Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.
Journal ArticleDOI
Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization
Hirotami Matsuo,Julien Chevallier,Nathalie Mayran,Isabelle Le Blanc,Charles Ferguson,Julien Fauré,Nathalie Sartori Blanc,Stefan Matile,Jacques Dubochet,Rémy Sadoul,Robert G. Parton,Francis Vilbois,Jean Gruenberg +12 more
TL;DR: It is found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multiveicular endosome membranes that exist where this lipid is found in vivo.
Journal ArticleDOI
On the preparation of cryosections for immunocytochemistry
TL;DR: The key preparation steps in the Tokuyasu thawed frozen section technique for immunocytochemistry, namely freezing, sectioning, thawing, and drying, were studied and it was indicated that glutaraldehyde-fixed, 2.1 M sucrose-infused pellets of cells were routinely vitrified by immersion in liquid nitrogen but water was crystallized when lower sucrose concentrations were used.
Journal ArticleDOI
Electron microscopy of frozen water and aqueous solutions
TL;DR: The structural and thermal properties of pure frozen water important for electron microscopy are summarized in an appendix as discussed by the authors, and a possible model for the mechanism of beam damage in aqueous solutions is proposed.