Showing papers by "Jaishree Jagirdar published in 1999"
TL;DR: The results indicate that CCAM is caused by an arrest in lung development, and, on the basis of cytodifferentiation, two major subtypes can be distinguished, and suggest that these two subtypes may arise at different stages of the branching of the bronchopulmonary tree.
71 citations
01 Jan 1999
TL;DR: In this article, the authors investigated the presence of human papilloma virus (HPV) sequences, loss of heterozygosity (LOH), and micro- satellite alterations (MAs) at five 3p chromosomal regions using 17 polymorphic markers in precisely microdissected archival tissues from 16 HIV-positive CINs and compared them with those present in 39 sporadic CIN cases.
Abstract: Objective. HIV infection is associated with an increased inci- dence of cervical malignancy and its precursor lesions (CIN, cer- vical intraepithelial neoplasia) compared with the general popu- lation. We studied the molecular abnormalities in the development of HIV-associated CIN and compared them with those present in CINs arising in HIV-indeterminate subjects ("sporadic CIN"). Methods. We investigated the presence of human papilloma virus (HPV) sequences, loss of heterozygosity (LOH), and micro- satellite alterations (MAs) at five 3p chromosomal regions using 17 polymorphic markers in precisely microdissected archival tissues from 16 HIV-positive CINs and compared them with those present in 39 sporadic CINs. Results. HPV sequences were detected in 36 of 55 (66%) CIN lesions, and high-risk oncogenic strains (HPV 16 and 18) ac- counted for 15 of them. No differences in the HPV frequencies were found between HIV-associated and sporadic CINs. Allelic losses at one or more chromosome 3p regions were frequently detected in CIN lesions (49%). The overall frequency of 3p LOH and the frequencies at all individual regions were similar in HIV- associated and sporadic CINs. The frequency of MA present in the HIV-associated CIN cases (0.093) was sixfold greater than in sporadic CINs (0.014; P 5 0.0001). At least 1 MA was present in 11 (69%) of 16 HIV-associated vs 5 of 39 (13%) sporadic CIN (P 5 0.0006). Molecular changes were independent of the presence of HPV sequences. Conclusion. Chromosome 3p deletions are frequently detected in the precursor lesions of cervical carcinoma (CIN) and there are no differences in the 3p LOH frequencies between HIV-associated and sporadic CIN lesions. Microsatellite alterations, which reflect widespread genomic instability, occur at greatly increased fre- quency in HIV-associated CIN. Although the mechanism under- lying the development of increased MAs is unknown, it may play a crucial role in the development of many HIV-associated neoplasias. © 1999 Academic Press
16 citations
TL;DR: Chromosome 3p deletions are frequently detected in the precursor lesions of cervical carcinoma (CIN) and there are no differences in the 3p LOH frequencies between HIV-associated and sporadic CIN lesions.
16 citations
TL;DR: It is concluded that TBNA through the flexible bronchoscope is safe and effective in the diagnosis of intrathoracic adenopathy in HIV-infected patients, and is particularly efficacious in the diagnoses of mycobacterial disease.
Abstract: Transbronchial needle aspiration (TBNA) of intrathoracic lymph nodes has been shown to be useful in the diagnosis and staging of bronchogenic carcinoma. With the exception of sarcoidosis, the usefulness of TBNA has not been widely investigated in other clinical settings. We investigated the utility of TBNA with a 19-gauge histology needle in HIV-infected patients with mediastinal and hilar adenopathy at Bellevue Hospital Center. We performed 44 procedures in 41 patients. Adequate lymph node sampling was obtained in 35 of 44 (80%), and diagnostic material was obtained in 23 of 44 (52%) procedures. TBNA was the exclusive means of diagnosis in 13 of 41 (32%) patients. Of the 44 procedures, 23 (52%) were performed in patients with mycobacterial disease, with TBNA providing the diagnosis in 20 of 23 (87%). In these patients, positive TBNA specimens included smears of aspirated materials for acid-fast bacilli in 11, mycobacterial culture in 14, and histology in 15. In other diseases, TBNA diagnosed sarcoidosis with noncaseating granulomata in 2 of 4 patients and non-small cell lung cancer in 1 of 2 patients. TBNA was not helpful in other diseases including Pneumocystis carinii pneumonia, infection with Cryptococcus or Nocardia, bacterial pneumonia, viral pneumonia, and Kaposi's sarcoma. No pulmonary diagnosis was established in five patients. No complications of TBNA occurred. We conclude that TBNA through the flexible bronchoscope is safe and effective in the diagnosis of intrathoracic adenopathy in HIV-infected patients, and is particularly efficacious in the diagnosis of mycobacterial disease. Furthermore, TBNA may provide the only diagnostic specimen in almost one-third of HIV-infected patients, thereby sparing these patients more invasive procedures such as mediastinoscopy.
10 citations
TL;DR: A case in which several localized granulomatous reactions developed at the facial injection sites 10 years after injection is presented, which is the longest lag period between collagen injection and onset of hypersensitivity reaction reported to date.
Abstract: Injectable bovine collagen (Zyderm, Zyplast; Collagen Corp., San Francisco, Calif.) for soft tissue augmentation has gained wide acceptance during the past 15 years. Both localized hypersensitivity reactions and rarer self-limited systemic reactions have been reported. The local granulomatous reaction has been studied histologically and was previously reported to occur up to 2.5 years after injection. 1 Clinically, these reactions manifest as localized erythema, induration, and swelling. We present a case in which several localized granulomatous reactions developed at the facial injection sites 10 years after injection. This is the longest lag period between collagen injection and onset of hypersensitivity reaction reported to date.
4 citations