Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been developed to determine submicro amounts of sugars and related substances. In conjunction with paper partition chromatography the method is useful for the determination of the composition of polysaccharides and their methyl derivatives.

Colorimetric Method for Determination of Sugars and Related Substances

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures

Publisher Summary This chapter discusses the assay of catalases and peroxidases are: (1) catalase assay by disappearance of peroxide; (2) method for crude cell extracts; (3) direct spectrophotometric assay of catalase and peroxidase in cells and tissues; and (4) peroxidase assay by spectrophotometric measurements of the disappearance of hydrogen donor or the appearance of their colored oxidation products. Two methods are described for the catalase assay by disappearance of peroxide are: ultraviolet spectrophotometry and permanganate titration. Ultraviolet spectrophotometryis a method devised, on the basis of the absorption curves for peroxide solutions, for determining the activity of catalase by direct measurements of the decrease of light absorption in the region 230 to 250 mμ caused by the decomposition of hydrogen peroxide by catalase. In the case of method for crude cell extracts, oxygen evolution caused by the decomposition of hydrogen peroxide is measured with the conventional manometric technique. Peroxidase assay by spectrophotometric measurements of the disappearance of hydrogen donor or the appearance of their colored oxidation products includes the guaiacol test and the pyrogallol test.

The assay of catalases and peroxidases.

[17] Amylases, α and β

The isolation and crystallization of the enzyme urease preliminary paper

A method is described for the colorimetric determination of phosphorus, using ferrous sulfate as a reducing agent. I wish to express my gratitude to the Rockefeller Foundation for financial assistance.

https://science.sciencemag.org/content/sci/100/2601/413.full.pdf

A method for the colorimetric determination of phosphorus.

The isolation and crystallization of the enzyme urease

Identification of Hemagglutinin of Jack Bean with Concanavalin A.

Chemistry and methods of enzmes , Chemistry and methods of enzmes , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

James B. Sumner

Papers

The isolation and crystallization of the enzyme urease preliminary paper

A method for the colorimetric determination of phosphorus.

The isolation and crystallization of the enzyme urease

Identification of Hemagglutinin of Jack Bean with Concanavalin A.

Chemistry and methods of enzymes