Showing papers by "James U. Bowie published in 1984"
TL;DR: It was concluded that reductive cleavage is an attractive method for determination of the structure of polysaccharides.
55 citations
TL;DR: It was suggested that the conversion of bovine alpha- to beta-thrombin does not affect the fibrinogen-binding site on thrombin, and differences in the reactivity of functional groups at the active site were explored.
23 citations
TL;DR: Structural features of mannan which participate in the formation of myeloperoxidase-mannan-neutrophil complexes have been studied by using a battery of structurally modified mannans to determine the mannan-neUTrophil interaction may involve cell membrane receptors for mannose.
Abstract: Structural features of mannan which participate in the formation of myeloperoxidase-mannan-neutrophil complexes have been studied by using a battery of structurally modified mannans. Mannan was isolated from Saccharomyces cerevisiae X2180 wild type and modified by strong alkaline degradation, selective (mild) alkaline degradation, and selective acetolysis. Mannose oligosaccharides bound to the peptide portion of mannan appeared to be required for binding of mannan to the neutrophil. The interaction of mannan with myeloperoxidase appeared to occur through phosphate groups of the mannan outer chain. The myeloperoxidase-mannan interaction was determined to be ionic in nature. The mannan-neutrophil interaction may involve cell membrane receptors for mannose.
20 citations
TL;DR: The independent synthesis of each of these derivatives, namely, 1,5-anhydro-2,3,4,6-tetra-O-methyl-, -2, 3, 4, 6-tentra- o-ethyl-, and -3,8-di-O.m.r. spectra, chemical-ionization (NH3) mass spectRA, and electron-impact mass spectra for all the derivatives are tabulated.
10 citations