J
Javier Santos-Aberturas
Researcher at John Innes Centre
Publications - 29
Citations - 1077
Javier Santos-Aberturas is an academic researcher from John Innes Centre. The author has contributed to research in topics: Streptomyces natalensis & Gene. The author has an hindex of 19, co-authored 28 publications receiving 826 citations. Previous affiliations of Javier Santos-Aberturas include University of Greifswald & University of León.
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Journal ArticleDOI
PimM, a PAS domain positive regulator of pimaricin biosynthesis in Streptomyces natalensis.
Nuria Antón,Javier Santos-Aberturas,Marta V. Mendes,Susana M. Guerra,Juan F. Martín,Jesús F. Aparicio +5 more
TL;DR: Results indicate that PimM plays its regulatory role independently of PimR, the first pathway-specific regulator of pimaricin biosynthesis, and the genes responsible for initiation and first elongation cycles of polyketide chain extension are among the major targets for regulation.
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Uncovering the unexplored diversity of thioamidated ribosomal peptides in Actinobacteria using the RiPPER genome mining tool.
Javier Santos-Aberturas,Govind Chandra,Luca Frattaruolo,Rodney Lacret,Thu H. Pham,Natalia M. Vior,Tom H. Eyles,Andrew W. Truman +7 more
TL;DR: RiPPER, a new tool for the family-independent identification of RiPP precursor peptides, is described and it is shown that previously undescribed RiPP gene clusters encoding YcaO and TfuA proteins are widespread in Actinobacteria and encode a highly diverse landscape of precursor peptide that are predicted to make thioamidated RiPPs.
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Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation
Jesús F. Aparicio,Eva G. Barreales,Tamara D. Payero,Cláudia M. Vicente,Antonio de Pedro,Javier Santos-Aberturas +5 more
TL;DR: The molecular genetics, uses, mode of action, analogue generation, regulation and strategies for increasing pimaricin production yields are described and discussed.
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Fully automatized high-throughput enzyme library screening using a robotic platform.
Mark Dörr,Michael P. C. Fibinger,Sandy Schmidt,Javier Santos-Aberturas,Dominique Böttcher,Anke Hummel,Clare Vickers,Moritz Voss,Uwe T. Bornscheuer +8 more
TL;DR: The fully automatized robotic platform enables proper monitoring and control of growth conditions in the microtiter plate format to ensure precise enzyme production for the interrogation of enzyme mutant libraries, protein stability tests and multiple assay screenings.
Journal ArticleDOI
Cholesterol Oxidases Act as Signaling Proteins for the Biosynthesis of the Polyene Macrolide Pimaricin
Marta V. Mendes,Eliseo Recio,Nuria Antón,Susana M. Guerra,Javier Santos-Aberturas,Juan F. Martín,Jesús F. Aparicio +6 more
TL;DR: Gene-inactivation and -complementation experiments revealed that pimE encodes a functional cholesterol oxidase and, surprisingly, that it is also involved in pimaricin biosynthesis, which broadens the scope of the biological functions for this type of oxidase.