Showing papers by "Jeremy N. Timmis published in 1998"
TL;DR: Northern blot analysis showed that the corresponding transcript is abundant in cotton fibre cells but absent in all other tissues tested, and that the gene is developmentally regulated during fibre elongation.
97 citations
TL;DR: Hypervariability of plastid sequence homologies was identified in the nuclear genomes of Spinacea oleracea and Beta vulgaris and individual plants were shown to possess a unique spectrum of nuclear sequences with ptDNA homology, apparently extended to somatic variation in B. vulgaris.
Abstract: A wide-ranging examination of plastid (pt)DNA sequence homologies within higher plant nuclear genomes (promiscuous DNA) was undertaken. Digestion with methylation-sensitive restriction enzymes and Southern analysis was used to distinguish plastid and nuclear DNA in order to assess the extent of variability of promiscuous sequences within and between plant species. Some species, such as Gossypium hirsutum (cotton), Nicotiana tabacum (tobacco), and Chenopodium quinoa, showed homogenity of these sequences, while intraspecific sequence variation was observed among different cultivars of Pisum sativum (pea), Hordeum vulgare (barley), and Triticum aestivum (wheat). Hypervariability of plastid sequence homologies was identified in the nuclear genomes of Spinacea oleracea (spinach) and Beta vulgaris (beet), in which individual plants were shown to possess a unique spectrum of nuclear sequences with ptDNA homology. This hypervariability apparently extended to somatic variation in B. vulgaris. No sequences with ptDNA homology were identified by this method in the nuclear genome of Arabidopsis thaliana.
73 citations