Genipin-crosslinked chitosan hydrogels as biomedical and pharmaceutical aids

Cell signaling pathways for the regulation of GATA4 transcription factor: Implications for cell growth and apoptosis

In this study, we examined the role of the miRNA miR-770-5p in cisplatin chemotherapy resistance in ovarian cancer (OVC) patients. miR-770-5p expression was reduced in platinum-resistant patients. Using a 6.128-fold in expression as the cutoff value, miR-770-5p expression served as a prognostic biomarker and predicted the response to cisplatin treatment and survival among OVC patients. Overexpression of miR-770-5p in vitro reduced survival in chemoresistant cell lines after cisplatin treatment. ERCC2, a target gene of miR-770-5p that participates in the NER system, was negatively regulated by miR-770-5p. siRNA-mediated silencing of ERCC2 reversed the inhibition of apoptosis resulting from miR-770-5p downreglation in A2780S cells. A comet assay confirmed that this restoration of cisplatin chemosensitivity was due to the inhibition of DNA repair. These findings suggest that endogenous miR-770-5p may function as an anti-oncogene and promote chemosensitivity in OVC, at least in part by downregulating ERCC2. miR-770-5p may therefore be a useful biomarker for predicting chemosensitivity to cisplatin in OVC patients and improve the selection of effective, more personalized, treatment strategies.

/pdf/mir-770-5p-inhibits-cisplatin-chemoresistance-in-human-2zgwpswvgy.pdf

MiR-770-5p inhibits cisplatin chemoresistance in human ovarian cancer by targeting ERCC2.

MiR-770-5p inhibits cisplatin chemoresistance in human ovarian cancer by targeting ERCC2

Microbial transformation of geniposide in Gardenia jasminoides Ellis into genipin by Penicillium nigricans

The aim of this study was to evaluate the role of GATA4 in hypertrophy and survival functions of CT-1 in the heart, and to apply chemical inhibitor strategies to assess a possible interaction of signaling pathways involved in these processes. Expression of GATA4 was determined at the mRNA expression (polymerase chain reaction, PCR) and protein binding activity (electrophoretic mobility shift assays, EMSAs) levels. Myocardial apoptosis was detected using the in-situ terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Parthenolide (an inhibitor to STAT3) and U0126 (an MEK inhibitor to block ERK1/2) were used to assess a possible interaction of signaling pathways involved in the processes. The results showed that CT-1 had a significant role in the expression of GATA4 mRNA and binding activity of cardiomyocytes. The stronger expression of GATA4 mRNA stimulated by CT-1 was essentially mediated by STAT3, and was negatively regulated by ERK1/2. The intercross relationship between STAT3 and ERK1/2 might assist CT-1 in cardiac hypertrophy. At the same time, CT-1 had its effect on anti-apoptosis and survival of cardiac myocytes. During this process, GATA4 expression showed a negative relationship with myocardial apoptosis. Obviously, STAT3 cannot affect the anti-apoptotic process of CT-1, but obstruction of ERK signaling pathway can inhibit the CT-1 anti-apoptotic effect significantly. Our data suggest that CT-1 can affect the expression of GATA4 mRNA and the binding activity of cardiac myocytes. GATA4 plays an important role in the hypertrophic effect of CT-1, in which STAT3 plays a primary role. The regulation of CT-1 on the anti-apoptotic process is mediated partly by GATA4, and can be inhibited by obstructing the ERK signaling pathway.

Relation of Cardiotrophin-1 (CT-1) and cardiac transcription factor GATA4 expression in rat's cardiac myocytes hypertrophy and apoptosis.

A new method for the simultaneous determination of genooposide and genipin in Gardenia jasminoides Eills by reversed-phase high perfomance liquid chromatography was studied.The method used a YWG-C 18 column,0 05 mol/L di-sodium hydrogen phosphate-methanol(72∶28)as the mobile phase at a flow rate of 1 0 ml/min with detection wavelengty at 240 nm.Retention times and recovery rates for geniposide and genipin were 6 33,9 49 min,and 99 10%,98 28%,respectively.The RSD of the results was 1 05% for geniposide,1 21% for genipin.The method was proved to be simple and reliable.

Jia Yu-jie

Papers

Relation of Cardiotrophin-1 (CT-1) and cardiac transcription factor GATA4 expression in rat's cardiac myocytes hypertrophy and apoptosis.

Simultaneous Determination of Geniposide and Genipin in Gardenia Jasminoides Eills by High Performance Liquid Chromatography