Showing papers by "Jian-Xun Liu published in 2006"

Protective effects of saponines of stem and leaf of Panax notoginseng on acute myocardial ischemia in anaesthetic dogs

Abstract: Objective To study the protective effects of saponines of stem and leaf of Panax notoginseng (PNSSL) on acute myocardial ischemia in anaesthetic dogs. Method The acute ischemia models were made by ligation of left anterior descending (LAD) artery. The myocardial blood flow (MBF) was determined by ultrasonic doppler. The experiments adopted epicardiogram mapping to measure the scope and degree of myocardial ischemia, quantitative histologic assay (nitroblue tetrazolium, N-BT stain) to determine the size of myocardial infarction. And the endothelin (ET) and thromboxane B2 (TXB2) were measured by radioimmunological assay. Result PNSSL was showed to obviously alleviate the degree of myocardial ischemia (sigma-ST) and narrow the ischemic area indicated by N-BT staining. In addition, PNSSL could increase the MBF of ischemia section. And the treatment could inhibit the ET and TXB2 release induced by ischemia and infarction. Conclusion PNSSL demonstrated to attenuate the damage subjected to myocardial ischemia and infarction, which may be due to its function of inhibiting the ET and TXA2 release, increasing the MBF, and then improving the damaged cardiac function.

[Study on time-effect and dose-effect of serum containing Chinese herbal medicine].

Abstract: OBJECTIVE To study the time-effect, dose-effect of serum containing Shuangshentongguan (SSTG), a formula composed by active fraction of Chinese medicine METHOD Serum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation LDH content in supernatant was detected after reoxygenation LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG RESULT The suppression rate of LDH release was not satisfied in serum containing high dose of SSTG There was no significant dose-effect relationship between them The suppression rate of LDH release was also different in drug serum taken at different time after SSTG treatment CONCLUSION It was not the case that the higher the dose given to animal, the better the pharmacological effects

Mechanism of serum containing SSTG on calcium overload in cardiomyocytes injuryed by hypoxia and reoxygenation

Abstract: OBJECTIVE To explore the effects and mechanism of Shuangshen Tongguan (SSTG) serum, a formula composed of the active fractions of Chinese medicine, on calcium overload in cultured cardiomyocytes injured by hypoxia and reoxygenation. METHOD The cardiomyocytes were deprived of oxygen and glucose to producl hypoxia reoxygenation injuried models. The changes of intracellular calcium fluorescence intensity induced by K+ and Thapsigargin were measured by fluorospectrophotometry and laster scanning confocal microscope respectively. RESULT Intracellular calcium concentration was low in normal cardiomyocytes and was enhanced after hypoxia/reoxygenation (P < 0.05); SSTC drug serum reduced the intracellular calcium concentration and depressed the increase of calcium fluorescence intensity in singular cardiomyocyte due to K+ and Thapsigargin stimulation. CONCLUSION Hypoxia/reoxygenation injury, K+ and Thapsigargin could induce calcium overload in cardiomyocytes. The effects of calcium antagonism of SSTG drug serum were achieved by inhibiting calcium inflow, promoting calcium re-absorption of calcium.