J
Joan Miquel Bernabé-Orts
Researcher at Polytechnic University of Valencia
Publications - 11
Citations - 386
Joan Miquel Bernabé-Orts is an academic researcher from Polytechnic University of Valencia. The author has contributed to research in topics: Cas9 & CRISPR. The author has an hindex of 6, co-authored 10 publications receiving 233 citations. Previous affiliations of Joan Miquel Bernabé-Orts include Spanish National Research Council & Institute of Science and Technology Austria.
Papers
More filters
Journal ArticleDOI
A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard
Marta Vazquez-Vilar,Joan Miquel Bernabé-Orts,Asun Fernández-del-Carmen,Pello Ziarsolo,José Blanca,Antonio Granell,Diego Orzaez +6 more
TL;DR: The genetic elements required for CRISPRs-based editing and transcriptional regulation were adapted to GoldenBraid (GB), a modular DNA construction framework being increasingly used in Plant Synthetic Biology, and a workflow for gRNAs construction was designed and optimized.
Journal ArticleDOI
Assessment of Cas12a‐mediated gene editing efficiency in plants
Joan Miquel Bernabé-Orts,Iván Casas‐Rodrigo,Eugenio G. Minguet,Viola Landolfi,Victor Garcia-Carpintero,Silvia Gianoglio,Marta Vazquez-Vilar,Antonio Granell,Diego Orzaez +8 more
TL;DR: It is shown that LbCas12a is a viable alternative to SpCas9 for plant genome engineering and no evidence of off‐target mutations neither in related sequences nor somewhere else in the genome is found.
Journal ArticleDOI
Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator.
Sara Selma,Joan Miquel Bernabé-Orts,Marta Vazquez-Vilar,Borja Diego-Martin,Maria Ajenjo,Victor Garcia-Carpintero,Antonio Granell,Diego Orzaez +7 more
TL;DR: A systematic comparison of 43 SunTag, SAM and scRNA-based TAD combinations tested for their ability to activate different promoters fused to a Luciferase reporter is shown.
Journal ArticleDOI
Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.
Fabio Pasin,Leonor C. Bedoya,Joan Miquel Bernabé-Orts,Araiz Gallo,Carmen Simón-Mateo,Diego Orzaez,Juan Antonio García +6 more
TL;DR: The pLX vectors as mentioned in this paper are a set of mini binary T-DNA plasmids suitable for Type IIS restriction endonuclease-and overlap-based assembly methods.
Journal ArticleDOI
A memory switch for plant synthetic biology based on the phage ϕC31 integration system
Joan Miquel Bernabé-Orts,Alfredo Quijano-Rubio,Marta Vazquez-Vilar,Javier Mancheño-Bonillo,Victor Moles-Casas,Sara Selma,Silvia Gianoglio,Antonio Granell,Diego Orzaez +8 more
TL;DR: A memory switch for whole plants based on the bacteriophage ϕC31 site-specific integrase is reported, built as a modular device made of standard DNA parts, designed to control the transcriptional state of two genes of interest by alternative inversion of a central DNA regulatory element.