Joel Crouzet

TL;DR: It is demonstrated that mutations in gyrA or mutations leading to reduced drug accumulation occur after alteration of GrlA, supporting the previous findings that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus.

TL;DR: Results indicated that the introduction of a targeting side chain moiety into the cationic lipid is permitted and the labeled lipopolyamines can be used to study the intracellular traffic of DNA/cationic lipid complexes.

TL;DR: The main result of this study was that the morphological and structural features of the lipopolyamine-DNA complexes did not depend on plasmid DNA length, while their gene transfer capacity was affected by the size of plasmids which were sandwiched between the lipid bilayers.

TL;DR: Minicircles are new DNA delivery vehicles which do not have such elements and are consequently safer as they exhibit a high level of biological containment, and exhibited a two- to 10-fold higher luciferase reporter gene activity than the unrecombined plasmid.

TL;DR: Two minicircles containing the luciferase or β-galactosidase gene under the control of the strong human cytomegalovirus immediate–early enhancer/promoter were produced in E. coli by site-specific recombination mediated by the phage λ integrase, which was used to excise the expression cassette from the unwanted plasmid sequences.