Ravi Rangara

TL;DR: Very efficient plasmid DNA transfer in muscle fibers is reported by using square-wave electric pulses of low field strength and of long duration by suggesting a direct effect of the electric field on DNA during electrotransfer.

TL;DR: Results indicated that the introduction of a targeting side chain moiety into the cationic lipid is permitted and the labeled lipopolyamines can be used to study the intracellular traffic of DNA/cationic lipid complexes.

TL;DR: The main result of this study was that the morphological and structural features of the lipopolyamine-DNA complexes did not depend on plasmid DNA length, while their gene transfer capacity was affected by the size of plasmids which were sandwiched between the lipid bilayers.

TL;DR: Very efficient luciferase gene transfer into muscle fibres is obtained through the delivery of square-wave electric pulses of moderate field strength and of long duration to muscle previously injected with plasmid DNA.

TL;DR: Two minicircles containing the luciferase or β-galactosidase gene under the control of the strong human cytomegalovirus immediate–early enhancer/promoter were produced in E. coli by site-specific recombination mediated by the phage λ integrase, which was used to excise the expression cassette from the unwanted plasmid sequences.