J
Joel Crouzet
Researcher at Rhône-Poulenc
Publications - 70
Citations - 4336
Joel Crouzet is an academic researcher from Rhône-Poulenc. The author has contributed to research in topics: Pseudomonas denitrificans & Plasmid. The author has an hindex of 38, co-authored 70 publications receiving 4225 citations.
Papers
More filters
Journal ArticleDOI
Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureus.
TL;DR: It is demonstrated that mutations in gyrA or mutations leading to reduced drug accumulation occur after alteration of GrlA, supporting the previous findings that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus.
Journal ArticleDOI
Synthesis, Activity, and Structure−Activity Relationship Studies of Novel Cationic Lipids for DNA Transfer
Gerardo Byk,Catherine Dubertret,Virginie Escriou,Marc Frederic,Gabrielle Jaslin,Ravi Rangara,Bruno Pitard,Joel Crouzet,Pierre Wils,Bertrand Schwartz,Daniel Scherman +10 more
TL;DR: Results indicated that the introduction of a targeting side chain moiety into the cationic lipid is permitted and the labeled lipopolyamines can be used to study the intracellular traffic of DNA/cationic lipid complexes.
Journal ArticleDOI
Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency
Patrick Kreiss,Philippe Mailhe,Daniel Scherman,Bruno Pitard,Béatrice Cameron,Ravi Rangara,Olivier Aguerre-Charriol,Marc Airiau,Joel Crouzet +8 more
TL;DR: The main result of this study was that the morphological and structural features of the lipopolyamine-DNA complexes did not depend on plasmid DNA length, while their gene transfer capacity was affected by the size of plasmids which were sandwiched between the lipid bilayers.
Journal ArticleDOI
A new DNA vehicle for nonviral gene delivery: supercoiled minicircle.
TL;DR: Minicircles are new DNA delivery vehicles which do not have such elements and are consequently safer as they exhibit a high level of biological containment, and exhibited a two- to 10-fold higher luciferase reporter gene activity than the unrecombined plasmid.
Journal ArticleDOI
Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer
Anne-Marie Darquet,Ravi Rangara,P Kreiss,Bertrand Schwartz,S Naimi,Pia Delaère,Joel Crouzet,Daniel Scherman +7 more
TL;DR: Two minicircles containing the luciferase or β-galactosidase gene under the control of the strong human cytomegalovirus immediate–early enhancer/promoter were produced in E. coli by site-specific recombination mediated by the phage λ integrase, which was used to excise the expression cassette from the unwanted plasmid sequences.