J
Johan Roeraade
Researcher at Royal Institute of Technology
Publications - 111
Citations - 3200
Johan Roeraade is an academic researcher from Royal Institute of Technology. The author has contributed to research in topics: Capillary electrophoresis & Mass spectrometry. The author has an hindex of 32, co-authored 111 publications receiving 3131 citations.
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Method for fabrication of microfluidic systems in glass
TL;DR: In this article, the authors describe a new way of fabricating integrated microfluidic elements in glass by employing a matrix of underpinning posts and a thin wall, surrounding etched flow channels, an efficient sealing of glass chips substrates to thin cover glass can be accomplished.
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Continuous segmented-flow polymerase chain reaction for high-throughput miniaturized DNA amplification.
Mario Curcio,Johan Roeraade +1 more
TL;DR: A continuous segmented-flow method for sequential DNA amplification is described in order to provide a basis for high-throughput genetic analysis and Perfluorodecalin was shown to be particularly suitable due to its hydrophobicity and inert properties.
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Protein microarrays for diagnostic assays.
Michael Hartmann,Michael Hartmann,Johan Roeraade,Dieter Stoll,Markus F. Templin,Thomas O. Joos +5 more
TL;DR: The current state of protein microarrays is reviewed and developments and future demands relating to protein arrays in their role as multiplexed immunoassays in the field of diagnostics are discussed.
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Improved capillary zone electrophoretic separation of basic proteins, using a fluorosurfactant buffer additive
TL;DR: In this paper, a new method to reduce the adsorption of basic proteins in capillary zone electrophoresis is described, where small amounts of a cationic fluorosurfactant are added to the running buffer.
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Automated microdroplet platform for sample manipulation and polymerase chain reaction
TL;DR: The system, which opens the route to strong reagents and labor savings in high-throughput applications, was validated on the clinically relevant quantification of progesterone receptor gene expression in human breast cancer cell lines.