Johannes Gumpert

TL;DR: This work compared levels of single-chain variable-fragment (scFv) production in Escherichia coliJM109 and P. mirabilis L VI, which express four distinct scFvs of potential clinical interest that show differences in levels of expression and in their tendencies to form aggregates upon periplasmic expression to find novel expression systems for recombinant antibodies.

TL;DR: A comparative analysis has been carried out with purified membranes of L-form cells, of parent vegetative hyphal cells, and of protoplasts derived from the latter, finding the phospholipid composition of the protoplast membrane differs quantitatively from that of the N form and the L form.

TL;DR: For the first time, cell wall-less L-form bacteria were used to establish such an alternative expression system and test its practicability, and the results showed that various recombinant proteins can be synthesized in considerable amounts as soluble, functionally active products with these cellwall-less strains.

TL;DR: A novel membrane surface display system that allows the anchoring of foreign proteins in the cytoplasmic membrane of stable, cell wall-less L-form cells of Escherichia coli and Proteus mirabilis is described and can provide a novel platform for numerous applications.

TL;DR: The new streptokinase shuttle plasmid, pMLS10 (7.3 kb), specified higher Skc yields in all hosts when compared to pSM752 constructed previously, and proved to be the most productive host.