After virtue, a study in moral theory

THE BETTER ANGELS OF OUR NATURE: WHY VIOLENCE HAS DECLINED. Steven Pinker, Penguin, New York, NY 2011. ISBN 978-0-670-02295-3.There's been a shooting in a Sikh Temple this morning. A lone gunman entered a Colorado theater and opened fire. Syrians are now engaged in civil war. Faced with daily news stories of death and destruction, it is easy to believe that things are getting worse. Not so, explains Harvard psychologist, Steven Pinker in his new work, The Better Angels of Our Nature: Why Violence has Declined.Pinker combines in-depth historical research with rigorous psychological research to argue the case for a decline in global violence. As Pinker aptly points out, many people look at our age as one of unprecedented violence and terror to be viewed with pessimism. Drawing on historical analysis, psychological research and findings from related sciences such as anthropology, sociology, and economics Pinker argues that the data paint a very different picture. In the first chapter, Pinker takes the reader on a quick journey through the history of the world pointing out that the ancient and medieval worlds were very different than the world we live in today. Numerous prehistoric skeletons bear evidence of very violent deaths. Ancient people destroyed entire tribes. Romans carried out violent executions. Medieval Knights led lies of violence and other Europeans meted out horrendous punishments for acts which might not even be judged worthy of condemnation in today's democracies. Finally, the early 20th century saw two World Wars before the long peace ensued. In light of that history, Pinker argues that perhaps we should reconsider our assumptions about our own world.In the first section of the book, Pinker identifies six historical trends which could have led to declines in violence. The first trend he calls the Pacification Process by which people gave up nomadic hunting and gathering lives for lives of agriculture in cities. Competition and anarchy in the prehistoric world made violence necessary for survival. The development of agriculture called for greater cooperation between individuals and the formation of governments to impose order created a world where violence was not always in one's best interest. Statistical analysis supports the idea that the emergence of states lead to a decline in violence. The second trend, the Civilizing Process, is an idea he developed from the work of Norbert Elias. In the late medieval and early modern periods, etiquette and social norms began to be important in social interactions, economics and technology began to advance, and governments began to become more centralized. This trend was also accompanied by a decline in violence. The third trend is the Humanitarian Revolution during which people began to increasingly find practices, such as torture, capital punishment, war and slavery, morally questionable. Empathy, compassion, and peace became important characteristics. The fourth trend is the Long Peace, which stems from the realization that since World War II no two major world powers have gone to war and, in spite of predictions to the contrary, nuclear weapons have never been used. …

The Better Angels of Our Nature: Why Violence Has Declined

Protein-free cell culture media supplements are described consisting of synergistic combinations of medium components, which when added to cell culture media, either serum supplemented or serum-free, enhance cell growth, culture longevity and product expression.

Cell culture medium for enhanced cell growth, culture longevity and product expression

Treatment methods for the remediation of nitroaromatic explosives.

Electrospinning, a common method for synthesizing 1D nanostructures, has contributed to developments in the electrical, electrochemical, biomedical, and environmental fields. Recently, a coaxial electrospinning process has been used to fabricate new nanostructures with advanced performance, but intricate and delicate process conditions hinder reproducibility and mass production. Herein, recent progress in new emerging parameters for successful coaxial electrospinning, and the various nanostructures and critical application areas resulting from these activities. Relationships between the new parameters and final product characteristics are described, new possibilities for nanostructures achievable via coaxial electrospinning are identified, and new research directions with a view to future applications are suggested.

Recent Progress in Coaxial Electrospinning: New Parameters, Various Structures, and Wide Applications

The replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum-free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum-free formulations and selection rules for the serum-free ingredients. In general, serum-free media which are “lipid rich” can sustain cell growth rates approaching that of serum supplemented cultures. Specific antibody secretion rate, however, is usually higher in serum-free media, irrespective of the lipid content.

Serum‐free media in hybridoma culture and monoclonal antibody production

A radial flow hollow fiber bioreactor has been developed that maximizes the utilization of fiber surface for cell growth while eliminating nutrient and metabolic gradients inherent in conventional hollow fiber cartridges. The reactor consists of a central flow distributor tube surrounded by an annular bed of hollow fibers. The central flow distributor tube ensures an axially uniform radial convective flow of nutrients across the fiber bed. Cells attach and proliferate on the outer surface of the fibers. The fibers are pretreated with polylysine to facilitate cell attachment and long-term maintenance of tissuelike densities of cell mass. A mixture of air and CO(2) is fed through the tube side of the hollow fibers, ensuring direct oxygenation of the cells and maintenance of pH. Spent medium diffuses across the cell layer into the tube side of the fibers and is convected away along with the spent gas stream. The bioreactor was run as a recycle reactor to permit maximum utilization of nutrient medium. A bioreactor with a membrane surface area of 1150 cm(2) was developed and H1 cells were grown to a density of 7.3 x 10(6) cells/cm(2).

A radial flow hollow fiber bioreactor for the large‐scale culture of mammalian cells

It has been cited in the literature on hollow fiber systems that pressure gradients persist, and the transmembrane flux of the hollow fiber system is dependent on the pattern of the pressure gradients. The pattern can be used to its advantage in immobilized enzyme systems. However, with immobilized living cell systems, the pressure gradients lead to a nonuniform environment within the hollow fiber cartridge and not necessarily favorable results. This article provides pertinent pressure-drop data on hollow fiber cartridges which are in flow configurations typical of immobilized cell culture work. The results illuminate operational problems that may arise in the culture of either anchorage dependent or independent cells. Possible solutions with crossflow systems are suggested.

Operation and pressure distribution of immobilized cell hollow fiber bioreactors

Physical and biochemical characterization of five commercial resins for immunoaffinity purification of factor IX.

An immunoaffinity process based on monoclonal antibody (MAb) to factor IX (FIX) has been developed. Initially, vitamin-K-dependent proteins from cryoprecipitate-poor plasma are isolated on DEAE-Sephadex. The eluate is applied to an immunoaffinity column that utilizes a divalent metal-ion-dependent MAb directed against FIX. After washing the column with high salt in the presence of magnesium ion, the FIX is eluted using a citrate- or EDTA-containing buffer. Coagulation assays and Western blots show no detectable amounts of any contaminating proteins. Purity of the FIX product is established using reduced and nonreduced Coomassie-stained SDS-PAGE and HPLC. The N-terminal 20 amino acids of the single peak of the HPLC were shown to be identical to those reported for FIX. The process shows no detectable leakage of monoclonal antibodies (MAb), efficient utilization of MAb, and provides yields greater than 95%. The use of solvent/detergent treatment as a potential viral inactivation methods is incorporated in the process. Studies with tritiated Triton X-100 indicate that the detergent can be washed out of the MAb column so that less than 1 ppm (total) Triton X-100 coelutes with the FIX.

John Tharakan

Papers

Serum‐free media in hybridoma culture and monoclonal antibody production

A radial flow hollow fiber bioreactor for the large‐scale culture of mammalian cells

Operation and pressure distribution of immobilized cell hollow fiber bioreactors

Physical and biochemical characterization of five commercial resins for immunoaffinity purification of factor IX.

Development of an immunoaffinity process for factor IX purification.