I have developed "tennis elbow" from lugging this book around the past four weeks, but it is worth the pain, the effort, and the aspirin. It is also worth the (relatively speaking) bargain price. Including appendixes, this book contains 894 pages of text. The entire panorama of the neural sciences is surveyed and examined, and it is comprehensive in its scope, from genomes to social behaviors. The editors explicitly state that the book is designed as "an introductory text for students of biology, behavior, and medicine," but it is hard to imagine any audience, interested in any fragment of neuroscience at any level of sophistication, that would not enjoy this book. The editors have done a masterful job of weaving together the biologic, the behavioral, and the clinical sciences into a single tapestry in which everyone from the molecular biologist to the practicing psychiatrist can find and appreciate his or

Principles of Neural Science

Microfluidic devices are finding increasing application as analytical systems, biomedical devices, tools for chemistry and biochemistry, and systems for fundamental research. Conventional methods of fabricating microfluidic devices have centered on etching in glass and silicon. Fabrication of microfluidic devices in poly(dimethylsiloxane) (PDMS) by soft lithography provides faster, less expensive routes than these conventional methods to devices that handle aqueous solutions. These soft-lithographic methods are based on rapid prototyping and replica molding and are more accessible to chemists and biologists working under benchtop conditions than are the microelectronics-derived methods because, in soft lithography, devices do not need to be fabricated in a cleanroom. This paper describes devices fabricated in PDMS for separations, patterning of biological and nonbiological material, and components for integrated systems.

Fabrication of microfluidic systems in poly(dimethylsiloxane)

基礎講座 電気泳動(Electrophoresis)

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Micro Total Analysis Systems. 1. Introduction, Theory, and Technology

Microsystems create new opportunities for the spatial and temporal control of cell growth and stimuli by combining surfaces that mimic complex biochemistries and geometries of the extracellular matrix with microfluidic channels that regulate transport of fluids and soluble factors. Further integration with bioanalytic microsystems results in multifunctional platforms for basic biological insights into cells and tissues, as well as for cell-based sensors with biochemical, biomedical and environmental functions. Highly integrated microdevices show great promise for basic biomedical and pharmaceutical research, and robust and portable point-of-care devices could be used in clinical settings, in both the developed and the developing world.

Cells on chips

We report a new approach for fabricating a fully integrated zero dead volume electrospray interface (ESI) on a glass CE microchip.

A New Approach for Fabricating a Zero Dead Volume Electrospray Tip for Non-Aqueous Microchip CE-MS

In this paper, we describe a microfluidic device with a novel two-layer m icrochannel structure to enable three-dimensional (3D) hydrodynamic focusing. Numerical results indicate that the device works reliably over a wide range of Reynolds numbers (Re). These results are confirmed using confocal laser scanning microscopy. The structure can be easily experimentally integrated into microfluidic systems with an optical module for cell/particle bioassay.

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Three-dimensional hydrodynamic focusing over a wide reynolds number range using a two-layer microfluidic design

A fluidic motherboard including fluidic and optical interconnections having modular functionality for optical analysis in microfluidic systems

Pesticides play a key factor in the high productivity achieved in modern agricultural food production. While increasing productivity and lowering production costs, they are potentially toxic and can have a serious impact on humans and the environment. In general, monitoring of pesticides and other environmental contaminants is performed in analytical laboratories, utilizing a multiplicity of time-consuming and cost-intensive chemical analysis methods like chromatography and mass spectrometry. To ensure food security and to monitor maximum residue levels in a highly globalized market, miniaturized analysis systems could provide inexpensive, portable devices for fast and reliable on-site monitoring of – not only – pesticides. Introduced already more than 20 years ago, lab-on-a-chip (LOC) devices found their way into biological and clinical research. Their fast analysis times, low sample volume and low reagent consumption are attractive for many applications in the life sciences, e.g., for DNA sequencing platforms and screening applications in drug development. It was only recently that the use of LOC systems gained considerable interest in the broad field of environmental analysis. In this work, several polymeric LOC systems for the analysis of dithiocarbamate (DTC) pesticides were designed and their performance was tested. Cyclic olefin polymer (COP) was studied as a potential material for non-aqueous analysis of DTC pesticides. While COP has some outstanding material properties compared to commonly used substrate materials, such as poly(methyl)methacrylate (PMMA) or polycarbonate (PC), it was shown that bonding of COP chips is challenging. Gold microband electrodes were integrated into microfluidic channels for electrochemical detection of the DTCs ziram and nabam. It was found that sulfur-containing DTC pesticides adsorb onto the gold surface of the electrode and thereby passivate it to a high extent. While sulfur-gold interactions of DTC pesticides were a major drawback for electrochemical detections, their high affinity for gold could

Design and Fabrication of Polymer-based Lab-on-a-Chip Devices Towards Applications in Food and Environmental Analysis

A miniaturized flow cytometry incorporating both fluidic and optical systems has a great possibility for portable biochemical sensing or point-of-care diagnostics. This paper presents a simple microfluidic flow cytometer combining reliable 3D hydrodynamic focusing and optical detection without optical fibers in a monolithic architecture fabricated by a single photolithographic process. The vertical flow focusing is achieved by the optimized inlet geometry in a PDMS lid onto the substrate with detection channel and integrated optics. The simplified approach indicates the possibility to be applied as a portable platform of cytometer chip.

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Jörg Peter Kutter

Papers

A New Approach for Fabricating a Zero Dead Volume Electrospray Tip for Non-Aqueous Microchip CE-MS

Three-dimensional hydrodynamic focusing over a wide reynolds number range using a two-layer microfluidic design

A fluidic motherboard including fluidic and optical interconnections having modular functionality for optical analysis in microfluidic systems

Design and Fabrication of Polymer-based Lab-on-a-Chip Devices Towards Applications in Food and Environmental Analysis

Simplified Monolithic Flow Cytometer Chip With Three-Dimensional Hydrodyanmic Focusing And Integrated Fiber-Free Optics