Showing papers by "Jörg Vogel published in 2001"
TL;DR: It is concluded that small RNAs are much more widespread than previously imagined and that these versatile molecules may play important roles in the fine-tuning of cell responses to changing environments.
786 citations
TL;DR: This review of Group II introns shows wide variation in both features and behaviour, and includes a focus on the diversity of evolutionary pathways taken.
287 citations
TL;DR: The data suggest that neither integrity of the dihydrouridine nor the anticodon domain is required for the nucleotidyltransferase-mediated addition of 3'-terminal CCA; interruption of these two structural elements by group II introns does not interfere with nucleotide-specific 5' maturation by RNase P; and processing intermediates of chloroplast tRNAs can be 3' polyadenylated.
Abstract: Higher plant chloroplasts provide the only experimentally validated example of functional tRNA genes that are disrupted by group II introns Here, precursor transcripts for tRNA(Gly)(UCC), tRNA(Val)(UAC), and tRNA(Ala)(UGC) were investigated for processing of 5' leader and 3' trailer sequences in vivo Use of intron-specific primer pairs and inclusion of a barley chloroplast splicing mutant specifically allowed us to evaluate the potential effect of intervening sequences that disrupt tRNA secondary and tertiary structures The data suggest that (1) neither integrity of the dihydrouridine nor the anticodon domain is required for the nucleotidyltransferase-mediated addition of 3'-terminal CCA; (2) interruption of these two structural elements by group II introns does not interfere with nucleotide-specific 5' maturation by RNase P; (3) processing intermediates of chloroplast tRNAs can be 3' polyadenylated; and (4) plastid DNA-encoded proteins are not required for 3' and 5' maturation of plastid tRNAs
20 citations