J
Jorja G. Henikoff
Researcher at Fred Hutchinson Cancer Research Center
Publications - 66
Citations - 21496
Jorja G. Henikoff is an academic researcher from Fred Hutchinson Cancer Research Center. The author has contributed to research in topics: Chromatin & Nucleosome. The author has an hindex of 46, co-authored 64 publications receiving 19163 citations. Previous affiliations of Jorja G. Henikoff include Memorial Sloan Kettering Cancer Center & University of Washington.
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Efficient transcription-coupled chromatin accessibility mapping in situ
TL;DR: By simply modifying the tagmentation conditions for histone H3K4me2/3 CUT&Tag, antibody-tethered tagmentation of accessible DNA sites is redirected to produce accessible DNA maps that are indistinguishable from the best ATAC-seq maps.
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Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation
TL;DR: This work shows that by simply modifying the tagmentation conditions for histone H3K4me2 or H3k4me3 CUT&Tag, antibody-tethered tagmentation of accessible DNA sites is redirected to produce chromatin accessibility maps that are indistinguishable from the best ATAC-seq maps.
Posted ContentDOI
Histone deposition pathways determine the chromatin landscapes of H3.1 and H3.3 K27M oncohistones
Jay F. Sarthy,Michael P. Meers,Derek H. Janssens,Jorja G. Henikoff,Shelli M. Morris,Matthew Biery,Heather Feldman,Patrick J. Paddison,Nicholas A Vitanza,James M. Olson,Kami Ahmad,Steven Henikoff +11 more
TL;DR: It is proposed that oncohistones inhibit the H3K27 methyltransferase as chromatin patterns are being duplicated in proliferating cells, predisposing them to tumorigenesis.
Posted ContentDOI
Profiling the epigenome at home
TL;DR: This work uses CUT&Tag@home with antibodies to trimethylated histone H3 lysine-4, −36, −27 and-9 to comprehensively profile the epigenome of human K562 cells, consisting respectively of active gene regulatory elements, transcribed gene bodies, developmentally silenced domains and constitutively silenced parasitic elements.
Posted ContentDOI
Epigenomic analysis of Formalin-Fixed Paraffin-Embedded samples by CUT&Tag
Steven Henikoff,Jorja G. Henikoff,Kami Ahmad,Derek H. Janssens,Zachary R Russell,Frank Szulzewsky,Sita Kugel,Eric C. Holland +7 more
TL;DR: In this article , a single-tube CUTAC protocol was proposed to produce high-resolution maps of RNA Polymerase II (RNAPII) at enhancers and promoters using FFPE samples.