José Luis Montesinos-Seguí

TL;DR: This review focuses on understanding the key factors that can enhance recombinant protein production in Pichia pastoris; they are the basis for a further discussion on future industrial applications with the aim of developing scalable alternative strategies that maximize yields and productivity.

TL;DR: This contribution aims to provide the state of the art of the different approaches that allow the design of rational strain and bioprocess engineering improvements in Pichia pastoris toward optimizing biop rocesses based on the results obtained in chemostat cultures.

TL;DR: The results validate that oxygen tension is essential to improve bioprocess efficiency in ROL production and enhances reliability of the system by keeping DO at optimal conditions.

TL;DR: Continuous operation with the novel constitutive promoter PGK is a realistic alternative to the standard fed-batch cultivation at a high cell density typically used in industrial bioprocesses for heterologous protein production in Pichia pastoris.

TL;DR: A versatile approach based on the physiological state of the yeast that can be used to implement the desired oxygen-limiting conditions to fermentations set-ups with different oxygen transfer capacities, alternative operating modes, and even for the production of other proteins of interest is presented.