J
Jose M. Guisan
Researcher at Spanish National Research Council
Publications - 498
Citations - 25913
Jose M. Guisan is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Immobilized enzyme & Lipase. The author has an hindex of 75, co-authored 490 publications receiving 23758 citations. Previous affiliations of Jose M. Guisan include University of Arkansas at Monticello & University of Strathclyde.
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Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
TL;DR: In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.
Journal ArticleDOI
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
Agatha Bastida,Pilar Sabuquillo,Pilar Armisén,Roberto Fernandez-Lafuente,Joan Huguet,Jose M. Guisan +5 more
TL;DR: These results suggest that lipases recognize these "well-defined" hydrophobic supports as solid interfaces and they become adsorbed through the external areas of the largeHydrophobic active centers of their "open and hyperactivated structure", which becomes a very promising immobilization method with general application for most lipases.
Journal ArticleDOI
Immobilization of lipases by selective adsorption on hydrophobic supports.
Roberto Fernandez-Lafuente,Pilar Armisén,Pilar Sabuquillo,Gloria Fernández-Lorente,Jose M. Guisan +4 more
TL;DR: Different approaches for lipase immobilization mainly related to the further use of immobilized derivatives to carry out enantio and regioselective hydrolysis in high water-activity systems are reviewed.
BookDOI
Immobilization of enzymes and cells
TL;DR: Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?
Journal ArticleDOI
Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases
Jose M. Palomo,Gloria Muñoz,Gloria Fernández-Lorente,Cesar Mateo,Roberto Fernandez-Lafuente,Jose M. Guisan +5 more
TL;DR: It seems that the “open structure” of lipases, adsorbed on hydrophobic supports, is much more active and much more stable than the corresponding “closed” structure even when the closed structure is undergoing a very intense multipoint covalent attachment.