J
Joseph Abatemarco
Researcher at University of Texas at Austin
Publications - 3
Citations - 244
Joseph Abatemarco is an academic researcher from University of Texas at Austin. The author has contributed to research in topics: Directed evolution & Aptamer. The author has an hindex of 3, co-authored 3 publications receiving 193 citations.
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Journal ArticleDOI
RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
Joseph Abatemarco,Maen F. Sarhan,James M. Wagner,Jyun-Liang Lin,Leqian Liu,Leqian Liu,Wafa Hassouneh,Wafa Hassouneh,Shuo-Fu Yuan,Hal S. Alper,Adam R. Abate,Adam R. Abate +11 more
TL;DR: RNA-aptamers-in-droplets (RAPID) is presented, a method that greatly expands the generality of ultrahigh-throughput microfluidic screening of millions of variants and combines aptamer-based fluorescent detection with droplet microfluidity to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.
Journal ArticleDOI
In vivo continuous evolution of genes and pathways in yeast.
TL;DR: This work synthetically optimize the retrotransposon Ty1 to enable in vivo generation of mutant libraries up to 1.6 × 107 l−1 per round, which is the highest of any in vivo mutational generation approach in yeast.
Journal ArticleDOI
Expanding the metabolic engineering toolbox with directed evolution
TL;DR: This review focuses on the various ways in which directed evolution can be applied in conjunction with metabolic engineering to improve product yields and discusses the current limitations in screening technology that hinder the full implementation of a metabolic pathway‐directed evolution approach.