J
Josephine E. Clark-Curtiss
Researcher at Arizona State University
Publications - 55
Citations - 2980
Josephine E. Clark-Curtiss is an academic researcher from Arizona State University. The author has contributed to research in topics: Mycobacterium leprae & Gene. The author has an hindex of 29, co-authored 54 publications receiving 2870 citations. Previous affiliations of Josephine E. Clark-Curtiss include Hebrew University of Jerusalem & University of Cologne.
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Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).
TL;DR: Methods described here, which have identified M. tuberculosis genes expressed in response to host interaction, will allow the study of gene expression in a variety of microorganisms, including expression resulting from interaction with human tissues in natural disease states.
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Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae
Richard A. Young,Vijay Mehra,Vijay Mehra,Douglas Sweetser,Thomas M. Buchanan,Josephine E. Clark-Curtiss,Ronald W. Davis,Barry R. Bloom +7 more
TL;DR: It is reported here that M. leprae specific epitopes recognized by all of 13 monoclonal antibodies tested were produced by recombinant phage in Escherichia coli, indicating that the leprosy bacillus contains five most immunogenic protein antigens.
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Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
TL;DR: Genomic libraries potentially representing more than 99.99% of each genome were prepared by cloning into the cosmid vector, pHC79, in Escherichia coli K-12, and Mycobacterium vaccae recombinant synthesized new polypeptides in minicells of E. coli, indicating that mycobacterial DNA can be expressed in E. bacteria.
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Induction of Mycobacterium avium gene expression following phagocytosis by human macrophages.
TL;DR: A procedure was developed to identify genes of M. avium that are specifically expressed when the bacilli are growing within macrophages that will be especially useful for identifying genes that are expressed in response to growth in specific environments from organisms with genetic systems that are not well characterized.
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Three Mycobacterium tuberculosis Rel Toxin-Antitoxin Modules Inhibit Mycobacterial Growth and Are Expressed in Infected Human Macrophages
TL;DR: This study characterized each Rel protein pair and established that they are functional TA modules, and determined that all six rel genes are expressed in broth-grown M. tuberculosis, whereas relE, relF, and relK are expressed during infection of human macrophages.