Showing papers by "Juan F. Ascaso published in 1999"

[Familial defect of apo B-100 in subjects with clinically diagnosed primary hypercholesterolemia: identification of the first family with this disorder in Spain].

Abstract: BACKGROUND: The aim of our study was to screen mutations responsible of FDB in subjects with primary hypercholesterolemia. MATERIAL AND METHODS: We have screened R3500Q and other mutations (PCR-SSCP analysis) in 110 subjects with primary hypercholesterolemia from the Valencia area (Spain), 95 of them with familial hypercholesterolemia (FH) and 15 with poligenic hypercholesterolemia (PHC). RESULTS: One out of 95 subjects carried the R3500Q mutation. We have searched in the family and have identified another affected subject. CONCLUSIONS: We have identified the first affected Spanish family from FDB. The prevalence of R3500Q mutations was of 1% in FH subjects in this series.

Insulin resistance and familial dyslipidaemias.

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Plasma Lp(a) values in familial hypercholesterolemia and its relation to coronary heart disease.

Abstract: BACKGROUND AND AIM To analyze plasma Lp(a) levels and examine different risk factors and coronary heart disease (CHD) in a sample of genetically diagnosed familial hypercholesterolemia (FH) patients. METHODS AND RESULTS Ninety heterozygous FH patients and 41 non-FH relatives were enrolled in a study to evaluate their plasma and lipoprotein cholesterol, as well as their triglyceride and Lp(a) levels. We found no differences in plasma Lp(a) levels and log transformed values between 90 FH subjects and their 41 unaffected relatives (22.3 mg/dl +/- 19.4 vs 17.7 mg/dl +/- 21.3 and 1.12 +/- 0.5 vs 0.96 +/- 0.54) nor between null allele and defective allele FH subjects (log Lp (a) levels 2.013 +/- 0.282 vs 1.959 +/- 0.151). FH CHD+ were significantly older, and had higher mean systolic and diastolic blood pressure and higher mean plasma triglyceride levels than FH CHD-. No differences in mean and log transformed Lp(a) plasma concentrations were found. CONCLUSIONS Plasma Lp(a) levels are not related to LDL receptor status and class mutations, nor to the presence of CHD in FH patients.

[Familial hypercholesterolemia and plasma Lp(a) levels: 2 cardiovascular risk factors].

Abstract: OBJECTIVE Lipoprotein (a) (Lp(a)) is a modified LDL particle in human plasma. Elevated Lp(a) plasma concentration has been associated with increase risk of premature heart disease in most cross-sectional studies. Familial hypercholesterolemia (FH) is a genetic disorder characterized by an elevation of LDL cholesterol (LDL-C) caused by molecular defects in the LDL receptor gene. The aim of our study is to analyze Lp(a) values in a genetic diagnosed FH group without coronary heart disease (CHD) and explain the considerable variation in clinical severity of FH patients. METHOD We have study plasma lipids and lipoprotein levels in 60 subjects with familial hypercholesterolemia without CHD and in 74 normolipidemic controls without personal history of CHD and dyslipidemia of the Valencia area in Spain. RESULTS We found differences in total and LDL cholesterol levels and apo B values as expected and also in plasma Lp(a) levels and log transformed values between FH subjects and normolipidemic controls (22.3 mg/dl +/- 19.4 vs 12.5 mg/dl +/- 12.6 p = 0.001 and 1.12 +/- 0.53 vs. 0.84 +/- 0.58 p = 0.008). The percentage of FH subjects with a cut point Lp(a) value > 20 mg/dl is significantly elevated in this group (47% vs 21% p = 0.002). Because of family relationships within the entire study population we also have compared 23 FH probands with the normolipidemic controls. Again the same results have been obtained (Lp(a) levels of 23.21 mg/dl +/- 19.2 vs 12.54 mg/dl +/- 12.63 p = 0.019 and log Lp(a) values of 1.19 +/- 0.42 vs 0.84 +/- 0.58 p = 0.01). CONCLUSION Our results indicate that FH subjects will have a more cardiovascular risk due to the potentiation of hypercholesterolemia and elevated Lp(a) values, indicating the addition effects of two different locus implicated in premature coronary heart disease and could explain the considerable variation in clinical severity of FH.