J
Jun Masuda
Researcher at Okayama University
Publications - 5
Citations - 418
Jun Masuda is an academic researcher from Okayama University. The author has contributed to research in topics: Homolysis & Hydrogen bond. The author has an hindex of 5, co-authored 5 publications receiving 397 citations.
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Journal ArticleDOI
A new mode of B12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase.
Naoki Shibata,Jun Masuda,Takamasa Tobimatsu,Tetsuo Toraya,Kyoko Suto,Yukio Morimoto,Noritake Yasuoka +6 more
TL;DR: This is the first crystallographic indication of the 'base-on' mode of cobalamin binding, which seems to favor homolytic cleavage of the cobalt-carbon bond and therefore to favor radical enzyme catalysis.
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How a protein generates a catalytic radical from coenzyme B12: X-ray structure of a diol-dehydratase–adeninylpentylcobalamin complex
TL;DR: The superimposition of the structure of the free coenzyme on that of enzyme-bound adeninylpentylcobalamin demonstrated that the tight enzyme-coenzyme interactions at both the cobalamin moiety and adenine ring of the adenosyl group would inevitably lead to cleavage of the cobalt-carbon bond.
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Substrate-induced conformational change of a coenzyme B12-dependent enzyme: crystal structure of the substrate-free form of diol dehydratase.
TL;DR: It was strongly suggested that the Co-C bond becomes largely activated (labilized) when the coenzyme binds to the apoenzyme even in the absence of substrate and undergoes homolysis through the substrate-induced conformational changes of the enzyme.
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Crystallization and preliminary X-ray study of two crystal forms of Klebsiella oxytoca diol dehydratase–cyanocobalamin complex
Jun Masuda,Tetsuya Yamaguchi,Takamasa Tobimatsu,Tetsuo Toraya,Kyoko Suto,Naoki Shibata,Yukio Morimoto,Yoshiki Higuchi,Noritake Yasuoka +8 more
TL;DR: Two crystal forms of Klebsiella oxytoca diol dehydratase complexed with cyanocobalamin have been obtained and preliminary crystallographic experiments have been performed, and some mercuric derivatives were found to be promising.
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Radical production simulated by photoirradiation of the diol dehydratase-adeninylpentylcobalamin complex.
TL;DR: Observations clearly indicate that homolysis of the Co-C bond of alkylcobalamin takes place upon illumination with visible light but is not readily cleaved during X-ray irradiation.