Showing papers in "Structure in 1999"

TL;DR: The structure of the heterodimeric Fe-only hydrogenase from Desulfovibrio desulfuricans is reported - the first for this class of enzymes and it is suggested that it was imported from the inorganic world as an already functional unit.

TL;DR: The structure shows how large ligands containing PC may be bound by CRP via a phosphate oxygen that projects away from the surface of the protein, inviting the design of inhibitors of CRP binding that may have therapeutic relevance to the possible role ofCRP in atherothrombotic events.

TL;DR: This study shows that the design of E2020 took advantage of several important features of the active-site gorge of AChE to produce a drug with both high affinity for A cholinesterase and a high degree of selectivity for A ChE versus butyrylcholinestersterase (BChE).

TL;DR: This paper presents the results of a large-scale parallel supercomputing experiment conducted at the North Carolina Supercomputing Center (NCSC) using the HL-06350 supercomputer, a state-of-the-art supercomputer developed at the National Institutes of Health (NIEHS) in 2015.

TL;DR: It is proposed that the unique alpha fingers might represent a common structural discriminator of the template-primer duplex that distinguishes between RNA and DNA during the replication of positive single-stranded RNA by viral RdRps.

TL;DR: The mode of binding of the inhibitor, and a comparison between the native and inhibited urease structures, indicate a novel mechanism for enzymatic urea hydrolysis which reconciles the available structural and biochemical data.

TL;DR: The heterolytic cleavage of molecular hydrogen seems to be mediated by the nickel center and the selenocysteine residue, and the putative oxo ligand is a signature of inactive 'unready' [NiFe] hydrogenases.

TL;DR: The structure of protein, lipid and water molecules in the crystals represents the functional entity of bR in the purple membrane of the bacteria at atomic resolution.

TL;DR: The scNS3-NS4A structure provides the first atomic view of polyprotein cis processing, and suggests autoinhibition and substrate-induced activation mechanisms for regulation of NS3 protease activity.

TL;DR: The recently determined structures of HIV-1 reverse transcriptase and Taq DNA polymerase in complex with DNA primer-template and an incoming nucleotide have shown that a large conformational change configures the polymerase active site for nucleotidyl transfer.

TL;DR: The OmpX structure shows that within a family of virulence-related membrane proteins, the membrane-spanning part of the protein is much better conserved than the extracellular loops.

TL;DR: The unusual ligand structure found in the oxidized form of D. vulgaris Miyazaki F [NiFe] hydrogenase was confirmed in the reduced form of the enzyme, with the exception that the electron density assigned to the monatomic sulfur bridge had almost disappeared.

TL;DR: This work has shown that N-glycosylation of a folded protein can have a significant stabilising effect on large regions of the backbone structure in glycoproteins.

TL;DR: A working model of the flap-opening mechanism in free HIV-1 protease which involves a transition from a semi-open to an open conformation that is facilitated by interaction of the Phe53 ring with the substrate is presented.

TL;DR: The polypeptide fold of NAP and the arrangement of the cofactors is related to that of Escherichia coli formate dehydrogenase (FDH) and distantly resembles dimethylsulphoxide reductase.

TL;DR: The recognition of Cys-tRNACys by EF-Tu-GDPNP is restricted to the aa-tRNA motif previously identified in Phe-TC and consists of the aminoacylated 3' end, the phosphorylated 5' end and one side of the acceptor stem and T stem.

TL;DR: The pocket at the interface of the two domains is probably the active site of the enzyme and there are sites on the second domain that may bind carbohydrate, as suggested by previously published kinetic data indicating that, in addition to the catalytic site, the enzyme has a second binding site specific for (1-->3, 1-->4)-beta-D-glucans.

TL;DR: The current state of knowledge of the α / β Hydrolase fold proteins is described, and a smaller definition of the required core and some possible future avenues of exploration are suggested.

TL;DR: The Atx1 structural motif represents a prototypical metal ion trafficking unit that is likely to be employed in a variety of organisms for different metal ions and is one of the largest unknown structures solved by direct methods.

TL;DR: Structural and biochemical data suggest that water-mediated interactions are as important as direct hydrogen bonds in the stability and specificity of recognition in protein-protein and protein-DNA interfaces.

TL;DR: In this paper, a computational procedure for calculating the depth of a residue from the protein surface is described. But the depth is often a more useful gage of residue burial than accessibility, probably due to the fact that the protein interior and surrounding solvent differ significantly in polarity and packing density.

TL;DR: This is the first crystallographic indication of the 'base-on' mode of cobalamin binding, which seems to favor homolytic cleavage of the cobalt-carbon bond and therefore to favor radical enzyme catalysis.

TL;DR: The cascade of phosphorylation-induced conformational changes in FixJN illustrates the role of conserved residues in stabilizing the phosphoryl group in the active site, triggering the structural transition and achieving the post-phosphorylation signaling events.

TL;DR: A systematic comparison of these databases has been carried out to determine their overall agreement in classifying protein structures, and extracts information from a consensus database, encompassing agreements between SCOP, CATH and FSSP.

TL;DR: This study is the first to compare the folding of structurally related proteins that are members of different superfamilies and suggests that the folding pathways of these immunoglobulin-like proteins share common features.

TL;DR: A reductionist approach, in which protein positions are classified by their local environments, has aided development of an appropriate energy expression.

TL;DR: A new canonical structure for the H1 loop of immunoglobulins, with c Ab-RN05 and cAb-Lys3 as reference structures is defined, which might also occur in human or mouse VH domains.

TL;DR: In this paper, the crystal structures of the wild-type PH domain and a gain-of-function mutant E41K in complex with D-myo -inositol 1,3,4,5-tetra-kisphosphate (Ins (1, 3, 4,5)P 4 ) were determined.

TL;DR: The tight binding of the tartrate ion conforms to the requirements of tight control of the reactive intermediates and suggests how the enzyme might use the substrate-binding energy to initiate cleavage of the cobalt-carbon bond.

TL;DR: This VEGFR2 kinase structure provides a target for design of selective anti-angiogenic therapeutic agents and may serve to properly orient the KID for signal transduction.