Showing papers by "Junta Sugiyama published in 2011"

Draft genome sequencing of the enigmatic basidiomycete Mixia osmundae.

Abstract: The massively parallel DNA sequencer is a powerful tool for the genome sequencing project. The sequencing is performed at random. So, even if we do not know the genome size or chromosome number of the target organism for sequencing, we can start to sequence the genome. On the other hand, a lot of sequence fragments are needed in order to assemble the fragments and obtain the complete genome sequence. Recently fungal genome sequences using massively parallel DNA sequencers have been published (DiGuistini et al., 2009; Martinez et al., 2009; Nowrousian et al., 2010). The aim of this article is to describe the draft genome sequence of the enigmatic fungus Mixia somundae and discuss the phylogeny. The phylum Basidiomycota consists of three subphyla Agaricomycotina, Pucciniomycotina, and Ustilaginomycotina (Hibbett et al., 2007). The subphylum Pucciniomycotina consists of eight classes (Hibbett et al., 2007). However, the phylogenetic relationships among the eight classes remain uncertain. The class Mixiomycetes is one of the eight classes. The class Mixiomycetes consists of a single order, Mixiales, and a single family, Mixiaceae (Bauer et al., 2006). The family Mixiaceae consists of a single genus, Mixia. The genus Mixia consists of a single species, Mixia osmundae, parasitic on Osmunda japonica (Japanese royal fern). Thus, at present, the class Mixiomycetes consists of only Mixia osmundae. We believe that this fungus is a key (essential) fungus to elucidate the phylogenetic relationships among the eight classes of Puccinomycotina. Nishida (1911) described Taphrina osmundae, based on two specimens collected in Japan. Mix (1947) added three specimens from Japan and also emended the description of Taphrina osmundae. Kramer (1958) proposed Mixia, a new genus typifi ed by Mixia osmundae (basionym: Taphrina osmundae Nishida) and tentatively placed the genus in the Protomycetaceae. Subsequently Kramer (1987) accommodated this genus in the new family Mixiaceae (Protomycetales). Recently the name Taphrina osmundae has been lectotypifi ed by Sugiyama and Katumoto (2008). On the other hand, based on evidence from the 18S rDNA sequence, morphological, and ultrastructural characters, Nishida et al. (1995) showed that Mixia osmundae is not a member of the Ascomycota, but a member of the Basidiomycota. Mixia osmundae has a unique phylogenetic position inferred from ribosomal RNA sequence comparison and a unique sporangium in the J. Gen. Appl. Microbiol., 57, 63‒67 (2011)

Saitoella S. Goto, Sugiyama, Hamamoto & Komagata (1987)

Abstract: Publisher Summary This chapter studies the genus Saitoella. The asexual reproduction is mainly by multipolar enteroblastic budding. The cells are ovoid to ellipsoidal, and occur singly or in pairs. True hyphae and well-differentiated pseudohyphae are absent. Ballistoconidia are not formed. In sexual reproduction it is found that a sexual state is unknown. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus in which sugars are not fermented, nitrate is assimilated, starch-like compounds are not synthesized, carotenoid pigments are formed, and xylose is absent in the cell walls. The diazonium blue B reaction is negative and the major ubiquinone system is Q-10. The type species taken is Saitoella complicata. The anamorphic genus Saitoella is discussed and summarized.