Showing papers by "Kamejiro Yamashita published in 1984"
TL;DR: In this article, the daily intake of 8.0 g per day of fructo-oligosaccharides for fourteen days was found to significantly reduce mean fasting blood glucose levels by 15 mg/dl, mean serum total cholesterol levels by 19 mg/dL and LDL-cholesterol levels by 17mg/dl in diabetic subjects.
271 citations
TL;DR: It is suggested that, in rats, active vitamin D preparations specifically inhibit PTH secretion without causing a significant increase in the serum calcium concentration, reflecting a direct feedback mechanism between active Vitamin D metabolite and the parathyroid glands.
Abstract: To study the effects of various vitamin D preparations on PTH secretion, serum calcium and urinary excretion of cAMP were monitored in conscious perfused rats, and the influences of a bolus iv injection of the preparations on these parameters were examined. Three hours after the administration of 0.25 μg/kg (0.6 nmol/kg) of 1α, 24 (R)-dihydroxycholecalciferol [1α, 24 (OH) 2D3], the urinary excretion of cAMP decreased to a level compatible with that of parathyroidectomized (PTX) rats (50% of initial value; p<0.05) with no change in the concentration of serum calcium (total and ionized). In PTX rats supplemented with bovine PTH (1 U/h), the vitamin D preparation showed no significant effects either on the urinary excretion of cAMP or on serum calcium. These effects were rather specific for active vitamin D preparations, i. e. 1α, 25 (OH) 2D3 (0.25μg/kg) and 1αOHD3 (1.25-6.25μg/kg). However, 24, 25 (OH) 2D3 (up to 25μg/kg) had no significant effect on these parameters. These results suggest that, in rats, active vitamin D preparations specifically inhibit PTH secretion without causing a significant increase in the serum calcium concentration, reflecting a direct feedback mechanism between active vitamin D metabolite and the parathyroid glands.
3 citations
TL;DR: The results suggest that glycosylation of hemoglobin in diabetic patients may be increased in various sites of the molecule in parallel with the blood glucose levels during the preceding 4-5 months.
Abstract: Hemoglobin (Hb) obtained from the hemolysate of normal subjects and diabetic patients was separated into HbA1a1, HbA1a2, HbA1b, HbA1c and HbA0 (major Hb) by Bio-Rex 70 cation exchange column chromatography. The glycosylated Hbs were further separated reproductively by cation exchange high performance liquid chromatography (HPLC), using 50mM sodium phosphate buffer pH 5.80 with 0-0.2M NaCl linear gradient system. HbA1b and HbA1c were separated into two subfractions (HbA1b1 and HbA1b2) and three subfractions (HbA1c1, HbA1c2, HbA1c3, ), respectively.The percentages of each subfraction except HbA1c1 in diabetic patients were significantly higher than those in normal subjects. Furthermore, HbA1c1, HbA1c2 and HbA1c3, correlated well with fasting blood glucose levels in the prior 5 month period, while subfractions in HbA1b revealed no significant correlation with blood glucose levels. The percentages of each subfraction of HbA1c in patients either with diabetic cataracts or with diabetic neuropathy were almost the same as those in the patients without complications. However, the percentages of each of the three groups were markedly higher than those of the normal subjects.These results suggest that glycosylation of hemoglobin in diabetic patients may be increased in various sites of the molecule in parallel with the blood glucose levels during the preceding 4-5 months.
3 citations
TL;DR: Investigations have demonstrated that the concentration of 5-phosphoribosyl l-pyrophosphate(PP-ribose-P) and the rate of purine biosynthesis de novo itself are increased by glucagon administered in mice and in isolated rat hepatocytes.
Abstract: Glucagon increases DNA synthesis in regenerating rat liverl or even in nonoperated rat liver2 and an elevated level of plasma glucagon has been documented after hepatectomy3 Other investigations have demonstrated that the concentration of 5-phosphoribosyl l-pyrophosphate(PP-ribose-P), which is an important regulator of purine biosynthesis de novo, and the rate of purine biosynthesis de novo itself are increased by glucagon administered in mice4 and in isolated rat hepatocytes5,6