K
Karla Mayolo-Deloisa
Researcher at Monterrey Institute of Technology and Higher Education
Publications - 33
Citations - 463
Karla Mayolo-Deloisa is an academic researcher from Monterrey Institute of Technology and Higher Education. The author has contributed to research in topics: PEGylation & Sepharose. The author has an hindex of 12, co-authored 28 publications receiving 393 citations. Previous affiliations of Karla Mayolo-Deloisa include University of Chile & Universidad Autónoma del Estado de Morelos.
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Journal ArticleDOI
Recovery of laccase from the residual compost of Agaricus bisporus in aqueous two-phase systems.
TL;DR: The results reported here demonstrated the potential application of ATPS for the valorisation of residual material and the potential establishment of a downstream process to obtain value added products with commercial application.
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Aqueous Two-Phase Systems at Large Scale: Challenges and Opportunities
TL;DR: This review work attempts to present the areas of opportunity to increase ATPS attractiveness at industrial levels and critically analyzes ATPS scale‐up strategies to enhance the potential industrial adoption.
Journal ArticleDOI
Separation of PEGylated from unmodified ribonuclease A using sepharose media
TL;DR: This study presents the use of a mildly amphiphilic support, Tris-capped CH Sepharose 4B as an alternative for separating PEGylated proteins from their unmodified counterparts.
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Hydrophobic interaction chromatography for purification of monoPEGylated RNase A
Karla Mayolo-Deloisa,Karla Mayolo-Deloisa,Ma. Elena Lienqueo,Barbara A. Andrews,Marco Rito-Palomares,Juan A. Asenjo +5 more
TL;DR: The purity of monoPEGylated RNase A is comparable to that obtained when the separation is performed using SEC, but the yield increases from 65% with SEC to ~85% with HIC, and this process represents a viable alternative for the separation of P EGylated proteins.
Journal ArticleDOI
Current advances in the non‐chromatographic fractionation and characterization of PEGylated proteins
Karla Mayolo-Deloisa,José González-Valdez,Daniel Guajardo-Flores,Oscar Aguilar,Jorge Benavides,Marco Rito-Palomares +5 more
TL;DR: This review presents current advances in some of the most widely used non-chromatographic strategies for the fractionation and analysis of PEG-protein conjugates.