Kazuhide Asakawa

TL;DR: The development of a targeted gene expression methodology in zebrafish based on the Tol2 transposable element and its application to the functional study of neural circuits is reported and discovered that targeted expression of TeTxLC in distinct populations of neurons in the brain and the spinal cord caused distinct abnormalities in the touch response behavior.

TL;DR: Through targeted photoconversion of U AS-driven Kaede and variegated expression of UAS-driven GFP in single cells, the cellular components of labeled circuits are characterized and the patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult.

TL;DR: Methodology for creating transgenic zebrafish using Tol2 vectors, and their applications to visualization and manipulation of specific tissues or cells in vivo and for functional studies of vertebrate neural circuits are outlined.

TL;DR: It is revealed that Tp53-mediated germ cell apoptosis induces sex reversal after the mutation of a DNA–repair pathway gene by compromising the survival of oocytes and suggests the existence of an oocyte-derived signal that biases gonad fate towards the female developmental pathway and thereby controls zebrafish sex determination.

TL;DR: The Gal4 gene trap and enhancer trap approaches together with various UAS lines should be important tools for investigating roles of genes and cells in vertebrate systems for a long time.