K
Kazuyuki Hoshijima
Researcher at University of Utah
Publications - 32
Citations - 2541
Kazuyuki Hoshijima is an academic researcher from University of Utah. The author has contributed to research in topics: Zebrafish & Gene. The author has an hindex of 22, co-authored 29 publications receiving 2269 citations. Previous affiliations of Kazuyuki Hoshijima include Tokyo Institute of Technology.
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Journal ArticleDOI
Simple Methods for Generating and Detecting Locus- Specific Mutations Induced with TALENs in the Zebrafish Genome
Timothy J. Dahlem,Kazuyuki Hoshijima,Michael J. Jurynec,Derrick Gunther,Colby G. Starker,Alexandra S. Locke,Allison M. Weis,Daniel F. Voytas,David Grunwald +8 more
TL;DR: Results presented here indicate the TALENs are highly sequence-specific and produce minimal off-target effects.
Journal ArticleDOI
Efficient identification of TALEN‐mediated genome modifications using heteroduplex mobility assays
Satoshi Ota,Yu Hisano,Michiko Muraki,Kazuyuki Hoshijima,Timothy J. Dahlem,David Grunwald,Yasushi Okada,Atsuo Kawahara +7 more
TL;DR: HMA is a rapid and sensitive analytical method for the detection of the TALEN‐mediated genome modifications at an endogenous locus and is easily estimated by the degree of multiple HMA profiles derived from TALen‐injected F0 embryos.
Journal ArticleDOI
Precise Editing of the Zebrafish Genome Made Simple and Efficient
TL;DR: Tagging donor sequences with reporter genes that can be subsequently excised improves recovery of edited alleles by an order of magnitude and facilitates recovery of recessive and phenotypically silent conditional mutations.
Journal ArticleDOI
Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish
Kazuyuki Hoshijima,Michael J. Jurynec,Dana Klatt Shaw,Ashley M. Jacobi,Mark A. Behlke,David Grunwald +5 more
TL;DR: Supernumerary guanine nucleotides at the 5' ends of single guide RNAs (sgRNAs) account for diminished CRISPR-Cas9 activity in zebrafish embryos and heritable deletion mutations of at least 50 kbp can be readily induced using pairs of duplex guide RNPs targeted to a single chromosome.
Journal ArticleDOI
Binding of the Drosophila transformer and transformer-2 proteins to the regulatory elements of doublesex primary transcript for sex-specific RNA processing.
TL;DR: UV-crosslinking experiments revealed that both female-specific transformer (tra) and transformer-2 (tra-2) products bind to the 13-nucleotide sequences of dsx pre-mRNA.