Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP. Keywords: primary biological atmospheric aerosol; climate; cloud condensation nuclei; biology; atmospheric ice nuclei (Published: 22 February 2012) Citation: Tellus B 2012, 64 , 15598, DOI: 10.3402/tellusb.v64i0.15598

https://www.tandfonline.com/doi/pdf/10.3402/tellusb.v64i0.15598

Primary biological aerosol particles in the atmosphere: a review

A review of atmospheric aerosol measurements

One of the most important parts of formulating a National Ambient Air Quality Standard is specifying the measurement methods for determining and attaining compliance. The samples taken for compliance are often pushed beyond their original purpose to identify sources, to evaluate the effectiveness of controls, and to determine relationships between pollution levels and public health. A full understanding of available sampling and analysis methods is needed, as well as an analysis of their costs, before acceptable monitoring approached can be specified. This review identifies issues related to measurement methods used to determine compliance with standards, describes current and future measurement methods and their limitations, and determines the extent to which existing technology can meet short-term and long-term needs for measuring compliance.

Measurement Methods to Determine Compliance with Ambient Air Quality Standards for Suspended Particles

The contribution of motorized traffic to air pollution is widely recognized, but relatively few studies have looked at the respiratory health status of subjects living near busy roads. We studied children in six areas located near major motorways in the Netherlands. We measured lung function in the children, and we assessed their exposure to traffic-related air pollution using separate traffic counts for automobiles and trucks. We also measured air pollution in the children's schools. Lung function was associated with truck traffic density but had a lesser association with automobile traffic density. The association was stronger in children living closest (< 300 m) to the motorways. Lung function was also associated with the concentration of black smoke, measured inside the schools, as a proxy for diesel exhaust particles. The associations were stronger in girls than in boys. The results indicate that exposure to traffic-related air pollution, in particular diesel exhaust particles, may lead to reduced lung function in children living near major motorways.

https://www.ademloos.be/sites/default/files/gezondheid_docs/airpoll.pdf

Air pollution from truck traffic and lung function in children living near motorways.

PM2.5 in China: Measurements, sources, visibility and health effects, and mitigation

An eight-stage cascade impactor, with cut-sizes ranging from 0.056 to 18 μm at a flow rate of 30 L/min, has been developed, calibrated, and tested in field programs. This impactor, called a microorifice uniform deposit impactor, has several features not normally found in cascade impactors. Ultrafine particles as small as 0.056 μm are collected while keeping the pressure drop moderate by utilizing multiple nozzles (up to 2000) as small as 52 μm in diameter. At each stage the collected particles are deposited uniformly over the entire impaction plate by rotating the impaction plate relative to the nozzles and by the correct radial placement of the nozzles. The particle cutoff characteristics (collection efficiency curves) of each stage and interstage losses were determined by means of monodisperse aerosols. The cutoff characteristics were found to be sharp and similar for all stages. This is Publication No. 758 from the Particle Technology Laboratory.

A Microorifice Uniform Deposit Impactor (MOUDI): Description, Calibration, and Use

Two single round nozzle impactors have been developed for use in Harvard’s indoor air pollution health study. Both impactors operate at flow rates of 4 L/m and are nearly identical, differing only in their cut sizes of 2.5 μm and 10 μm aerodynamic diameters. Two identical cascaded stages of the same cut size are used to obtain sharp cut-off characteristics. The particles are deposited on impaction plates made of oil impregnated, porous material to reduce particle bounce and are discarded. Only the particles collected on the afterfilter are analyzed. Special care has been taken to collect the particles uniformly on the afterfilter to aid in particle analysis. The jmpactors were calibrated with a vibrating orifice monodisperse aerosol generator. However, due to the sharp cut of the impactors, doublets and triplets in the calibration aerosols, even in small quantities, gave erroneous calibration curves. Therefore, the number of doublets and triplets in the challenging aerosols were measured and appropriate c...

Low Flow Rate Sharp Cut Impactors for Indoor Air Sampling: Design and Calibration

A cascade impactor has been developed that is suitable for personal sampling. The impactor can be used with four, six, or eight stages with cut points ranging from 0.5 µm to 21 µm aerodynamic diameter. Although the impactor can be operated over a fairly wide flow rate range, it is designed specifically to be operated at 2 L/min so that a personal sampler pump may be used as the air mover. The nozzles of the impactor are radial slots with six slots per stage for the first six stages. Circular nozzles arranged in a radial pattern are used for the last two stages. On each stage, the area between the nozzles is used as the impaction surface for the stage before it. This design allows for the impactor to be compact, rugged and lightweight (170 gm for the four-stage design). The impactor stages are contained in a mounting bracket that can be clipped to the lapel or pocket. Although the impactor is designed for personal use, it also can be used as a compact impactor for general sampling tasks. The particle cut-o...

A personal cascade impactor: design, evaluation and calibration.

Electrostatic effects in aerosol sampling and filtration

A coating technique for applying uniform, thin layers of grease onto impactor substrates is described. The technique involves dissolving or diluting the grease in a solvent, applying the grease solution to the substrate, the spinning the substrate at a high speed. The coating thickness is controlled by the concentration of grease in the solution and by the spin speed. Apiezon-L vacuum grease and silicone oil were tested with this technique. Coating thicknesses between 0.3 and 9 μm were successfully generated on the polycarbonate film. The coated substrates and uncoated polycarbonate were tested for particle collection efficiency with polystyrene latex in two stages (0.56- and 1.0-μm cut-size) of a microorifice uniform deposit impactor. In the range of coating thicknesses tested, the collection efficiency of Apiezon-L was strongly related to the coating thickness; large coating thicknesses greatly improved particle collection. For the silicone oil, collection efficiency was high for all the coating thickne...

Kenneth L. Rubow

Papers

A Microorifice Uniform Deposit Impactor (MOUDI): Description, Calibration, and Use

Low Flow Rate Sharp Cut Impactors for Indoor Air Sampling: Design and Calibration

A personal cascade impactor: design, evaluation and calibration.

Electrostatic effects in aerosol sampling and filtration

Effect of Coating Thickness on Particle Bounce in Inertial Impactors