Kenton L. Lohman

TL;DR: The sensitivity and specificity of the real-time PCR assay were directly compared with those of the current standard for detection of influenza virus: virus isolation in embryonated chicken eggs and hemagglutinin subtyping by hemagGLutination inhibition (HI) assay.

TL;DR: It is demonstrated that the AIV and H7 RRT-PCR assays are good tools for the rapid screening of flocks and LBMs.

TL;DR: Streptococcus pneumoniae was used as a model organism to develop and refine a real-time fluorescence PCR assay and enhanced DNA purification method and will make it adaptable to identification of many bacterial pathogens and provide potential for adaptation to direct detection from patient specimens.

TL;DR: PCR findings were consistent with culture results and were confirmed by direct nucleotide sequencing, which confirmed the presence of Salmonella enterica serotype Panama in 109 attendees of a church picnic in Kerr County, Texas in June 2001.

TL;DR: The results confirm that this assay is a highly sensitive and timely surveillance tool for rapid detection and simultaneous subtyping of clinical influenza specimens isolated worldwide.