Showing papers in "Journal of Clinical Virology in 2002"

TL;DR: This work has recently completed the evaluation of a sequence-directed modification of a primer set for NLV RT-PCR detection, and it is shown that this modification can be used to validate a complete assay validation, using a broad range of genetically distinct viruses.

TL;DR: Technological improvements have given the ability to develop and introduce systems for most viruses of clinical interest, and to obtain clinical relevant information needed for optimal antiviral treatment options, and it also becomes more feasible to introduce molecular testing for those viruses that can easily be detected using classical virological methods, like culture techniques or antigen detection.

TL;DR: It is proposed that induction of IE-1 gene expression is the first step in reactivation of the virus in an immunocompromised transplant recipient, and that it occurs as a result of the allogeneic response, which induces expression of TNF and subsequent activation of NFkappaB, and ischemia/reperfusion injury, which induce activation of AP-1.

TL;DR: It is shown that certain mutations in either the S or polymerase gene produce functionally significant changes in the respective overlapping gene that may produce HBV mutants with altered antigenicity and/or replication and a natural history that may be distinctly different to wild type HBV.

TL;DR: Viruses from two genera, the Norwalk like viruses and Sapporo like viruses, are a common cause of acute, nonbacterial gastroenteritis in humans and are an important public health issue in Europe and worldwide.

TL;DR: This study shows that HCII high-risk probe cocktail detects at least 15 HPV genotypes not included in the current HCIIHigh risk probe cocktail, including genotypes currently considered to be low-risk HPVs.

TL;DR: It is concluded that mCMV establishes true molecular latency rather than a low-level persistence of productive infection, based on two recent advances on a highly improved assay of infectivity and on very sensitive RT PCRs for detecting viral transcripts during latency.

TL;DR: Cytomegalovirus infections are likely to remain a significant cause of morbidity and mortality among immunosuppressed patients and the design of improved diagnostic techniques and the development of antiviral agents are required.

TL;DR: A generally broad testing for different herpesviruses from CSF samples is highly recommended and determination of the virus DNA level in CSF by quantitative assays seems to be of high importance for elucidating aspects concerning the prognosis of disease, the prediction of distinct CNS manifestations, and possibly the differentiation between specific virus-associated disease and unspecific presence of virus inCSF, especially in immunocompromised patients.

TL;DR: Comparison of the genomes from classic versus AIDS-associated KSHV in the US indicates that it was derived originally by reactivation and spread of a subset of the endogenous viruses carried by descendants of immigrants from endemic areas of Northern and Eastern Europe, the Mediterranean and sub-Saharan Africa.

TL;DR: The role of VZV infections in CNS complications seems remarkable, often presenting without rash, and even these cases should be promptly recognized in order to conduct proper antiviral therapy.

TL;DR: The role of CSF PCR in the diagnosis and therapeutic management of herpesvirus infections of the nervous system, particularly those caused by HSV and VZV is summarized.

TL;DR: It is confirmed that the real-time enterovirus RT-PCR assay for CSF specimens is significantly more sensitive than viral culture.

TL;DR: The results suggest that virus isolation is a useful and sensitive technique for confirmation of dengue virus infection, especially when serum samples are collected before fever subsides.

TL;DR: Investigation of post transplant HHV-6 and -7 antigenemia was in relation to symptomatic CMV disease after liver transplantation supported the suggestion that CMV, HHV -7 and -8 may have interactions.

TL;DR: It is indicated that routine use of PCR will enhance the number and speed with which viral respiratory tract infections are diagnosed in lung transplant recipients.

TL;DR: A robust PCR was developed, with very small inter-run variation, and the sensitivity seems to be good or excellent for orf in humans, but asymptomatic virus shedding in sheep or goats may complicate veterinary applications of the assay.

TL;DR: RT-PCR testing for EVM is an important tool to aid in the diagnosis of children with non-specific febrile illness and should translate into significant health care savings, as measured by shortened patient stays.

TL;DR: In this paper, the authors developed and evaluated methods based on nucleic acid sequence based amplification (NASBA) for detection of enterovirus sequences in clinical samples, and compared them with reverse-transcribed PCR (RT-PCR) and virus culture.

TL;DR: The detection of viral DNA by PCR technique has become the "gold standard" method for laboratory diagnosis of herpesvirus infections of CNS and may be useful to confirm the diagnosis retrospectively.

TL;DR: The selection of the initial regimen requires consideration of several factors, including baseline viral load and CD4 count, drug pharmacokinetics, potency, tolerability, safety, resistance and salvageability.

TL;DR: Progress has been made in molecular characterization of GPCMV, and insights from these studies should prove germane to the understanding of the correlates of protective immunity for the fetus in vaccine studies, and should assist in prioritization of vaccine strategies in HCMV vaccine trials.

TL;DR: The results show dramatic improvement in specificity, presumably due to a generally greater expertise of participating laboratories, more frequent use of enzymatic or mechanical contamination control mechanisms, and increased utilization of standardized reagents (commercial kits).

TL;DR: It is suggested that calicivirus and rotavirus are major agents of gastroenteritis in children younger than 3 years of age in Buenos Aires, Argentina and the introduction of affordable viral diagnosis in pediatric hospitals will improve patient care by reducing the unnecessary use of antibiotics.

TL;DR: At birth, virus load in urine seems to be superior to that in cord vein blood to reflect the situation in the organs precisely, and the significant decrease of virus Load in children with therapy may reflect the efficacy of therapy.

TL;DR: The results of these studies showed that the Bayer Assay has excellent reproducibility, a broad linear range, throughput of 168 patient results per two-plate run in a 22-h period, and few limitations for use, making it an attractive method for general laboratory use of monitoring HIV-1-infected patients.

TL;DR: To the knowledge, this is the first reported case of HPEV1 infection related to encephalomyelitis, and it is shown that human parechoviruses can be responsible for severe central nervous system infections in children.

TL;DR: There is a linear time-dependent appearance of HBV mutations for lamivudine in the authors' population and in a minority of patients (5%), development of a mutation was followed by a significant elevation of serum transaminases.

TL;DR: The assay detailed here has proved an invaluable tool for the investigation of acute gastroenteritis in specimens from patients of all ages, particularly where less sensitive assays such as EM are being employed for diagnosis.

TL;DR: I have been given the task of looking into a crystal ball to see if there is a formula for a CMV vaccine, and six questions will be discussed: does natural immunity protect, is the induction of antibodies important, isThe induction of T helper cell responses important, how should aCMV vaccine be constructed, and finally, is vaccine development worthwhile?