Molecular marker for discriminating cytoplasmic male sterility of Pyrus spp. and uses thereof

The present invention relates to a primer set for use for cultivar discrimination in pear; and uses thereof. The primer set of the present invention may be used to discriminate pear cultivars based on their maternal line. A primer set composition according to the present invention may include a primer set represented by a base sequence of SEQ ID NO:1 and SEQ ID NO:2, and since a select pear cultivar and a cultivar bred from the same as a maternal line can be accurately distinguished using the primer set composition according to the present invention, it is possible to select desirable cultivars from seedlings being distributed.

A composition for cultivar discrimination in pear

Molecular marker for predicting fruit shape of pear and use thereof

The present invention relates to a marker for discriminating a cultivar of a Hongro apple and a bud mutation thereof, and a use thereof. When using the marker of the present invention, it is possible to quickly and accurately discriminate the cultivar of the Hongro apple and the bud mutation thereof which do not show a large difference in the phenotype due to high genetic similarity. Thus, the present invention can contribute to the protection of intellectual property rights and promotion of branding of domestic cultivars of the Hongro apple and the bud mutation thereof and can also be used to secure competitiveness in related industries.

Marker for discriminating Hongro apple and its bud mutation cultivar and use thereof

The present invention relates to a sequence-specific amplification polymorphism (S-SAP) marker for discriminating Fuji apples and bud mutation cultivars thereof, and a use thereof. When the marker of the present invention is used, it is possible to quickly and accurately discriminate between Fuji apples and the bud mutation cultivars thereof, which do not show a large difference in phenotype due to high genetic similarity. Therefore, the marker can contribute to intellectual property rights protection and branding promotion of domestic Fuji apples and the bud mutation cultivars thereof in the future, and can be used to secure competitiveness of related industries.

S-SAP S-SAP marker for discriminating Fuji apple and its bud mutation cultivar and use thereof

The present invention relates to CAPS markers for discriminating apple cultivars and to uses thereof, wherein the CAPS markers derived from single nucleotide polymorphism (SNP) selected through a genotyping-by-sequencing (GBS) technique exhibit accurate polymorphism for eight apple cultivars of Fuji, Gala, Golden delicious, Earlyblaze, Hongo, Kent, Aikanokaori and Jonahgold, and thus the eight kinds of apple cultivars can be effectively discriminated and can be applied very usefully in molecular breeding research and apple breeding industry of the apple cultivars.

CAPS CAPS markers for discriminating of apple cultivars and uses thereof

The present invention relates to a CAPS marker for discriminating the presence or absence of a flowerpot and its use. The present invention makes a linkage map and implements a kruskal-wallis test about an F1 mating seedling cultivated by cross-breeding a mini-pear as a pollen parent and a golden-pear as an ovary parent, thereby making a gene map about the pollen presence of the pollen in the pear. Through the present invention, a specific gene locus involved in the presence or absence of the pollen of the pear is checked to check the gene map about the presence or absence of the pollen in the pear for supplying direct information on the genetic background of presence or absence of the pollen in the pear. In addition, the present invention can be very useful in molecular breeding research and breeding industry of the pear by developing a primer for selection of molecular markers of the traits of the presence or absence of the pollen in the pear with reference to a mark close to a location of the high density gene map bout the pollen presence of the pollen in the pear.

CAPS CAPS marker for discriminating presence or absence of pollen in pear and uses thereof

The present invention relates to a molecular marker for distinguishing a golden pear variety and a mini-pear variety More specifically, the insertion or deletion (InDel) molecular marker, which is developed through using next generation sequencing (NGS) base sequence data of the golden pear and mini-pear varieties, is used to analyze polymorphism and distinguish a golden pear variety and a mini-pear variety According to the present invention, the InDel market based on the NGS base sequence data of the golden pear and mini-pear varieties have excellent identification functions regarding the golden pear and mini-pear varieties, determine the polymorphism of the eastern pears and hybrids thereof, and is used to write pear DNA profiles to be utilized in various fields such as pear variety determination, variety protection, and gene mapping

Markers for discrimination of Whangkeumbae and Minibae

The present invention relates to a composition for the production of a GBS library of fruit crop genomic DNA comprising ApeKI and TfiI restriction enzymes, a method for constructing the GBS library of the fruit crop including a step of cutting the genomic DNA of the fruit crop with the ApeKI and TfiI restriction enzymes, and a GBS library constructed by the method.

Kim Dae Il

Papers

CAPS CAPS markers for discriminating of apple cultivars and uses thereof

CAPS CAPS marker for discriminating presence or absence of pollen in pear and uses thereof

Markers for discrimination of Whangkeumbae and Minibae

Molecular marker for discriminating cytoplasmic male sterility of Pyrus spp. and uses thereof

GBS Optimized restriction enzyme combination for constructing Pyrus spp. genotyping by sequencing library