Showing papers by "Klaus Palme published in 1987"

A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis.

Abstract: A fast and simple extraction procedure of plant tissue for two-dimensional gel electrophoresis is presented. The procedure is especially useful for the extraction of plant cell suspension cultures, callus and other plant tissues having a high content of phenol oxidases, polysaccharides, polynucleotides, terpenoids and other substances interfering with isoelectric focusing. Due to the speed of the extraction procedure (about 20 min), large numbers of samples containing only milligram amounts of tissue can be easily processed. The simplicity of the method makes it particularly suitable for the extraction of radiolabeled tissues (35S, 32P). This method is perfectly compatible with silver staining, autoradiography and Western blotting analysis.

Protein Phosphorylation in Membranes of Nicotiana Tabacum

Abstract: We have identified growth related changes in phosphoproteins from Nicotiana tabacum membranes. Changes in the phosphorylation patterns suggest that different substrates and different phosphorylation sites in substrates are used. Most remarkably, a change in the phosphorylation of a 33.000 Da phosphoprotein was correlated with the presence of cytokinin-like growth hormones in the culture media. A membrane bound protein kinase with an apparent Mr of 16.500 was isolated from Nicotiana tabacum and purified to homogeneity.