K
Koichiro Murashima
Researcher at University of California, Davis
Publications - 39
Citations - 1356
Koichiro Murashima is an academic researcher from University of California, Davis. The author has contributed to research in topics: Clostridium cellulovorans & Cellulase. The author has an hindex of 18, co-authored 39 publications receiving 1304 citations.
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Journal ArticleDOI
Cellulosomes from mesophilic bacteria
TL;DR: Plant cell wall-degrading enzymes have become increasingly important, since the development of efficient biomass degradation methods and the conversion of sugars to valuable products and utilizable forms of energy could lead to less dependence on imported petroleum as a fuel and chemical source.
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Purification and characterization of new endo-1,4-β-d-glucanases from Rhizopus oryzae
Koichiro Murashima,Tomoko Nishimura,Yuko Nakamura,Jinichiro Koga,Tastuki Moriya,Naomi Sumida,Takashi Yaguchi,Toshiaki Kono +7 more
TL;DR: New extracellular endoglucanases, designated RCE1 and RCE2, produced by Rhizopus oryzae isolated from the soil were purified to apparent homogeneity from the culture supernatant, indicating a new type of endoglucaanases having the lowest optimum temperature among the family 45 endoglUCanases.
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Heterologous Production of Clostridium cellulovorans engB, Using Protease-Deficient Bacillus subtilis, and Preparation of Active Recombinant Cellulosomes
TL;DR: The full-length EngB containing the dockerin domain was expressed by Bacillus subtilis WB800, to prevent the proteolytic cleavage of the enzymatic subunit between the catalytic and dockerin domains that was observed in previous attempts to express EngB with Escherichia coli.
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Synergistic Effects of Cellulosomal Xylanase and Cellulases from Clostridium cellulovorans on Plant Cell Wall Degradation
TL;DR: Although synergistic effects on corn cell wall degradation were found in simultaneous reactions with XynA and cellulases, no synergism effects were observed in sequential reactions.
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Synergistic Effects on Crystalline Cellulose Degradation between Cellulosomal Cellulases from Clostridium cellulovorans
TL;DR: The mixture of the cellulosomes containing ExgS and EngH showed higher activity and synergy degrees than the other cellulosome mixtures, indicating the synergistic effect between EngH and Exg S was the most dominant effect among the three mixtures for crystalline cellulose degradation.