K
Konstantin Pervushin
Researcher at Nanyang Technological University
Publications - 107
Citations - 7449
Konstantin Pervushin is an academic researcher from Nanyang Technological University. The author has contributed to research in topics: Transverse relaxation-optimized spectroscopy & Protein structure. The author has an hindex of 34, co-authored 103 publications receiving 6849 citations. Previous affiliations of Konstantin Pervushin include University of Basel & Hoffmann-La Roche.
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Attenuated T2 relaxation by mutual cancellation of dipole–dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution
TL;DR: The TROSY principle should benefit a variety of multidimensional solution NMR experiments, especially with future use of yet somewhat higher polarizing magnetic fields than are presently available, and thus largely eliminate one of the key factors that limit work with larger molecules.
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TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins
TL;DR: The present paper shows that the implementation of transverse relaxation-optimized spectroscopy ([15N,1H]-TROSY) into triple resonance experiments results in several-fold improved sensitivity for 2H/13C/ 15N-labeled proteins and approximately twofold sensitivity gain for 13C/15N- labeled proteins.
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Trosy-type triple-resonance experiments for sequential nmr assignments of large proteins
TL;DR: Salzmann et al. as mentioned in this paper implemented transverse relaxation-optimized spectroscopy (TROSY) in the four triple resonance experiments (15 N, 1 H)-TROSy-HN(CO)CA, ( 15 N, 2 H/13 C/15 N-labeled protein gyrase 23B), which achieved a 3-fold sensitivity gain for each of the four experiments.
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NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy
Konstantin Pervushin,Akira Ono,César Fernández,Thomas Szyperski,Masatsune Kainosho,Kurt Wüthrich +5 more
TL;DR: NMR observation of 15N---15N and 1H--- 15N scalar couplings across the hydrogen bonds in Watson-Crick base pairs in a DNA duplex, hJNN and hJHN represents new parameters of interest for both structural studies of DNA and theoretical investigations into the nature of the hydrogen Bonds.
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Transverse Relaxation-Optimized Spectroscopy (TROSY) for NMR Studies of Aromatic Spin Systems in 13C-Labeled Proteins
TL;DR: The TROSY improvement in signal-to-noise relative to corresponding conventional NMR experiments increases with increasing molecular size and can be further significantly enhanced by combined use of the 1H and 13C steady-state magnetizations.