K
Kotikanyadanam Sreekrishna
Researcher at Phillips Petroleum Company
Publications - 2
Citations - 276
Kotikanyadanam Sreekrishna is an academic researcher from Phillips Petroleum Company. The author has contributed to research in topics: Pichia pastoris & Gene expression. The author has an hindex of 2, co-authored 2 publications receiving 275 citations.
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Journal ArticleDOI
High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris.
Kotikanyadanam Sreekrishna,Nelles L,Potenz R,Cruze J,Mazzaferro P,Fish W,Fuke M,Holden K,Phelps D,Wood P +9 more
TL;DR: In this paper, human tumor necrosis factor (TNF) alpha/cachectin was expressed in the methylotrophic yeast Pichia pastoris at high levels (greater than 30% of the soluble protein) by placing the TNF cDNA under the control of regulatory sequences derived from the alcohol oxidase gene.
Journal ArticleDOI
High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris
Kotikanyadanam Sreekrishna,Rica Helayne Potenz,John A. Cruze,William Richard Mccombie,Kathryn Ann Parker,Lynn P. Nelles,Paul K. Mazzaferro,Kathy A. Holden,Roger G. Harrison,Phyllis J. Wood,Doris A. Phelps,Cindi E. Hubbard,Motohiro Fuke +12 more
TL;DR: TNF contained in P. pastoris cell lysates was biologically active as determined by its cytotoxic effect on murine L‐929 fibroblast cells and the bioactivity was retained for at least 6 months in the lysate stored frozen at −20 °C in the presence of protease inhibitor PMSF.