Kris Barreto

TL;DR: 89Zr-DFO-nimotuzumab had low organ absorbed dose and effective dose that makes it suitable for potential human use and there was no apparent normal tissue toxicity as shown by cell blood counts and blood biochemistry analyses at 168-fold and 25-fold excess of the projected human radioactive and mass dose of the agent.

TL;DR: A new way to construct recombinant antibody‐like “ devices” by using a bottom‐up approach to build them from well‐behaved discrete recombinant antibodies domains or “parts”, thereby creating devices with desired properties based on summed properties of parts and in configurations that cannot be obtained by using genetic engineering.

TL;DR: An NGS-assisted antibody discovery platform by integrating phage-displayed, single-framework, synthetic Fab libraries is developed and a rapid and simple method for linking and sequencing all diversified CDRs in phage Fab pools is developed.

TL;DR: Results highlight the ease and utility of using the modular SpyTag/SpyCatcher protein ligase system for site-specific fluorescent labeling of protein-based imaging probes and confirm the affinity and specificity of the IRDye800CW-labeled imaging probes.

TL;DR: The utility of using antibody fragments to optimize clearance, tumor labeling, and biodistribution properties for developing anti-HER3 probes for image-guided surgery or PET imaging is highlighted.