Showing papers by "Krystyna Klimaszewska published in 2003"
TL;DR: The activity levels of G US varied widely among the individual translines of P. mariana, and there was a strong indication that the logarithm of GUS activity increased with the number of gus copies, in lines with one to five known insertions (uncensored).
Abstract: Seventy transgenic tissue lines (translines) of three spruce species (Picea mariana, P. glauca and P. abies) were characterized with respect to the integration pattern of the gus (β-glucuronidase) gene, and the level of GUS activity was determined in 81 lines. The majority of the P. mariana translines (18 lines of 22) integrated multicopies of the transgene, whereas mostly single integrations were detected in the other two species. The activity levels of GUS varied widely among the individual translines of P. mariana, and there was a strong indication that the logarithm of GUS activity increased with the number of gus copies (P=0.0003) in lines with one to five known insertions (uncensored). The average level of GUS activity, in lines that integrated one gene copy, was the highest in white spruce followed by black spruce and Norway spruce (22.7, 16.5 and 6.3 nmol 4-methylumbelliferone min-1 mg-1 protein, respectively).
28 citations
15 Jan 2003
TL;DR: This chapter discusses the history of embryogensis, the process ofogenesis and Embryogenesis, and strategies for applying SE in Industry.
Abstract: Introduction
Basic Techniques
Basal Media
Plant Material
Surface disinfection, Excision of Explants and Culture
Incubation Environment
Clonal Propagation
General Considerations
Organogenesis and Embryogenesis
Strategies for applying SE in Industry
Haploid Culture and Somatic Hybridization
Genetic Engineering
Conclusion
Bibliography
Keywords:
embryogensis;
growth promoters;
culture medium