K
Kurt James Cox
Researcher at Massachusetts Institute of Technology
Publications - 2
Citations - 592
Kurt James Cox is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Stable isotope labeling by amino acids in cell culture. The author has an hindex of 2, co-authored 2 publications receiving 404 citations.
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Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.
Victoria Hung,Namrata D. Udeshi,Stephanie S Lam,Ken H. Loh,Kurt James Cox,Kayvon Pedram,Steven A. Carr,Alice Y. Ting,Alice Y. Ting +8 more
TL;DR: This protocol describes the generation of an appropriate APEX2 fusion construct, proteomic sample preparation, and mass spectrometric data acquisition and analysis, and a two-state stable isotope labeling by amino acids in cell culture (SILAC) protocol is used for proteomic mapping of membrane-enclosed cellular compartments.
Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2
Victoria Hung,Namrata D. Udeshi,Stephanie S Lam,Ken H. Loh,Kurt James Cox,Kayvon Pedram,Steven A. Carr,Alice Y. Ting,Alice Y. Ting +8 more
TL;DR: In this article, an engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest, and upon the addition of hydrogen peroxide for 1 min to cells preloaded with a biotin-phenol substrate, the peroxide generates biotinphenoxyl radicals that covalently tag proximal endogenous proteins.