Kyle Curtis

TL;DR: Three RT-ddPCR assays were used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in weekly samples from nine WWTPs in southeastern Virginia, and fluctuations in population normalized loading rates agreed with known outbreaks during the study.

TL;DR: Examining the variability of SARS-CoV-2 concentrations in wastewater grab samples collected every 2 hours for 72 hours compared with corresponding 24-hour flow-weighted composite samples suggests that grab samples may be sufficient to characterize Sars-CoVs, but additional calculations using these data may be sensitive to grab sample variability and warrant the use of flow- Weighted composite sampling.

TL;DR: Cumulative probability analyses showed that multiple samples from a single location can provide >95% level of confidence in positive and negative results, suggesting that when multiple samples are necessary to account for in situ variability, a single marker can yield sufficiently reliable results.

TL;DR: The CSI-MST approach is explained and several case study examples of how this approach can be adapted to different scale watersheds to identify potential sewer infrastructure issues are provided.

TL;DR: The importance of drainage basin sediments as they enhance the persistence and transport of the fecal indicator bacteria E. coli within a watershed is investigated and an indirect control of water column bacterial concentration by sediment type and erodibility is suggested.