Platelet-derived growth factor

Effects of TNFα on verocytotoxin cytotoxicity in purified human glomerular microvascular endothelial cells

https://www.kidney-international.org/article/S0085-2538(15)34226-5/pdf

Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice

Organ-derived microvessel endothelial cells exhibit differential responsiveness to thrombin and other growth factors.

Rat mesangial cells (MC) release a factor that competes in a dose-dependent manner with 125I-labeled platelet-derived growth factor (PDGF) for binding to human foreskin fibroblasts (HFF). The competitor activity in mesangial cell conditioned medium (MCCM) is reversible, trypsin sensitive, and inhibited by anti-PDGF IgG. MCCM also expresses potent mitogenic activity to HFF. Anti-PDGF IgG, in concentrations that completely abolished the mitogenic activity of pure PDGF and the competitor activity of MCCM, only partially (33-41%) inhibits this mitogenic activity. The PDGF receptor competing activity as well as the total mitogenic activity, coelutes with labeled pure PDGF on Sephacryl S-200 gel chromatography. Cation exchange chromatography of concentrated MCCM yields a major mitogen peak with little competitor activity and a smaller mitogenic peak with comparable competitor activity, suggestive of the presence of other mitogens in MCCM besides the PDGF-like protein. PDGF is a potent mitogen and may play a role at inflammatory sites. The production of PDGF-like protein by MC may provide insights for understanding the pathogenesis of glomerular diseases.

/pdf/production-of-platelet-derived-growth-factorlike-protein-by-2c2kk0tve7.pdf

Production of platelet-derived growth factorlike protein by rat mesangial cells in culture

Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE).

https://rupress.org/jem/article-pdf/160/1/323/1094741/323.pdf

L J Striker

Papers

Isolation, characterization, and propagation in vitro of human glomerular endothelial cells.